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 Previous Article

Journal of Bacteriology, December 2007, p. 8765-8768, Vol. 189, No. 23
0021-9193/07/$08.00+0     doi:10.1128/JB.01215-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Amino Acid Toxicities of Escherichia coli That Are Prevented by Leucyl-tRNA Synthetase Amino Acid Editing{triangledown}

Vrajesh A. Karkhanis,{dagger} Anjali P. Mascarenhas, and Susan A. Martinis*

Department of Biochemistry, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave., 419 Roger Adams Laboratory, Box B-4, Urbana, Illinois 61801-3732

Received 27 July 2007/ Accepted 7 September 2007

Leucyl-tRNA synthetase (LeuRS) has evolved an editing function to clear misactivated amino acids. An Escherichia coli-based assay was established to identify amino acids that compromise the fidelity of LeuRS and translation. Multiple nonstandard as well as standard amino acids were toxic to the cell when LeuRS editing was inactivated.

* Corresponding author. Mailing address: Department of Biochemistry, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave., 419 Roger Adams Laboratory, Box B-4, Urbana, Illinois 61801-3732. Phone: (217) 244-2405. Fax: (217) 244-5858. E-mail: martinis{at}life.uiuc.edu

{triangledown} Published ahead of print on 21 September 2007.

{dagger} Present address: Program in Gene Function and Expression, University of Massachusetts Medical School, Aaron Lazare Medical Research Building-670C, 364 Plantation Street, Worcester, MA 01605-4321.

Journal of Bacteriology, December 2007, p. 8765-8768, Vol. 189, No. 23
0021-9193/07/$08.00+0     doi:10.1128/JB.01215-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sikora, M., Jakubowski, H. (2009). Homocysteine editing and growth inhibition in Escherichia coli. Microbiology 155: 1858-1865 [Abstract] [Full Text]  
  • Boniecki, M. T., Vu, M. T., Betha, A. K., Martinis, S. A. (2008). From the Cover: CP1-dependent partitioning of pretransfer and posttransfer editing in leucyl-tRNA synthetase. Proc. Natl. Acad. Sci. USA 105: 19223-19228 [Abstract] [Full Text]  
  • Yao, P., Zhou, X.-L., He, R., Xue, M.-Q., Zheng, Y.-G., Wang, Y.-F., Wang, E.-D. (2008). Unique Residues Crucial for Optimal Editing in Yeast Cytoplasmic Leucyl-tRNA Synthetase Are Revealed by Using a Novel Knockout Yeast Strain. J. Biol. Chem. 283: 22591-22600 [Abstract] [Full Text]  


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