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Journal of Bacteriology, December 2007, p. 9044-9049, Vol. 189, No. 24
0021-9193/07/$08.00+0     doi:10.1128/JB.01264-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

A Horizontal Gene Transfer Event Defines Two Distinct Groups within Burkholderia pseudomallei That Have Dissimilar Geographic Distributions{triangledown} ,{dagger}

Apichai Tuanyok,1 Raymond K. Auerbach,1 Thomas S. Brettin,2 David C. Bruce,2 A. Christine Munk,2 J. Chris Detter,2 Talima Pearson,1 Heidie Hornstra,1 Rasana W. Sermswan,3 Vanaporn Wuthiekanun,4 Sharon J. Peacock,4 Bart J. Currie,5 Paul Keim,1 and David M. Wagner1*

Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona 86011-5640,1 Los Alamos National Laboratory, Los Alamos, New Mexico 87545,2 Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand,3 Wellcome Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand,4 Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia5

Received 6 August 2007/ Accepted 13 September 2007

Burkholderia pseudomallei is the etiologic agent of melioidosis. Many disease manifestations are associated with melioidosis, and the mechanisms causing this variation are unknown; genomic differences among strains offer one explanation. We compared the genome sequences of two strains of B. pseudomallei: the original reference strain K96243 from Thailand and strain MSHR305 from Australia. We identified a variable homologous region between the two strains. This region was previously identified in comparisons of the genome of B. pseudomallei strain K96243 with the genome of strain E264 from the closely related B. thailandensis. In that comparison, K96243 was shown to possess a horizontally acquired Yersinia-like fimbrial (YLF) gene cluster. Here, we show that the homologous genomic region in B. pseudomallei strain 305 is similar to that previously identified in B. thailandensis strain E264. We have named this region in B. pseudomallei strain 305 the B. thailandensis-like flagellum and chemotaxis (BTFC) gene cluster. We screened for these different genomic components across additional genome sequences and 571 B. pseudomallei DNA extracts obtained from regions of endemicity. These alternate genomic states define two distinct groups within B. pseudomallei: all strains contained either the BTFC gene cluster (group BTFC) or the YLF gene cluster (group YLF). These two groups have distinct geographic distributions: group BTFC is dominant in Australia, and group YLF is dominant in Thailand and elsewhere. In addition, clinical isolates are more likely to belong to group YLF, whereas environmental isolates are more likely to belong to group BTFC. These groups should be further characterized in an animal model.

* Corresponding author. Mailing address: Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011-5640. Phone: (928) 523-0686. Fax: (928) 523-0639. E-mail: Dave.Wagner{at}nau.edu

{triangledown} Published ahead of print on 12 October 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, December 2007, p. 9044-9049, Vol. 189, No. 24
0021-9193/07/$08.00+0     doi:10.1128/JB.01264-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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