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Journal of Bacteriology, February 2007, p. 1176-1178, Vol. 189, No. 3
0021-9193/07/$08.00+0     doi:10.1128/JB.01628-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Role of Phosphoenolpyruvate in the NADP-Isocitrate Dehydrogenase and Isocitrate Lyase Reaction in Escherichia coli{triangledown}

Tadashi Ogawa,1 Keiko Murakami,1 Hirotada Mori,2,3 Nobuyoshi Ishii,2 Masaru Tomita,2 and Masataka Yoshin1,2*

Department of Biochemistry, Aichi Medical University School of Medicine, Nagakute, Aichi 480-1195,1 Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0035,2 Nara Institute of Science and Technology, Ikoma, Nara 630-0101, Japan3

Received 20 October 2006/ Accepted 15 November 2006

Phosphoenolpyruvate inhibited Escherichia coli NADP-isocitrate dehydrogenase allosterically (Ki of 0.31 mM) and isocitrate lyase uncompetitively (Ki' of 0.893 mM). Phosphoenolpyruvate enhances the uncompetitive inhibition of isocitrate lyase by increasing isocitrate, which protects isocitrate dehydrogenase from the inhibition, and contributes to the control through the tricarboxylic acid cycle and glyoxylate shunt.

* Corresponding author. Mailing address: Department of Biochemistry, Aichi Medical University School of Medicine, Aichi 489-1195, Japan. Phone: 81-52-264-4811. Fax: 81-561-61-4056. E-mail: yoshino{at}aichi-med-u.ac.jp.

{triangledown} Published ahead of print on 1 December 2006.

Journal of Bacteriology, February 2007, p. 1176-1178, Vol. 189, No. 3
0021-9193/07/$08.00+0     doi:10.1128/JB.01628-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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