This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nguyen, K.-A.
Right arrow Articles by Potempa, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nguyen, K.-A.
Right arrow Articles by Potempa, J.

 Previous Article  |  Next Article 

Journal of Bacteriology, February 2007, p. 833-843, Vol. 189, No. 3
0021-9193/07/$08.00+0     doi:10.1128/JB.01530-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Does the Importance of the C-Terminal Residues in the Maturation of RgpB from Porphyromonas gingivalis Reveal a Novel Mechanism for Protein Export in a Subgroup of Gram-Negative Bacteria?{triangledown} ,{dagger}

Ky-Anh Nguyen,1* James Travis,1 and Jan Potempa1,2

Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 30602,1 Department of Microbiology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30-387 Krakow, Poland2

Received 1 October 2006/ Accepted 15 November 2006

The mature 507-residue RgpB protein belongs to an important class of extracellular outer membrane-associated proteases, the gingipains, from the oral pathogen Porphyromonas gingivalis that has been shown to play a central role in the virulence of the organism. The C termini of these gingipains along with other outer membrane proteins from the organism share homologous sequences and have been suggested to function in attachment of these proteins to the outer membrane. In this report, we have created a series of truncated and site-directed mutants of the C terminus from a representative member of this class, the RgpB protease, to investigate its role in the maturation of these proteins. Truncation of the last two residues (valyl-lysine) from the C terminus is sufficient to create an inactive version of the protein that lacks the posttranslational glycosylation seen in the wild type, and the protein remains trapped behind the outer membrane. Alanine scanning of the last five residues revealed the importance of the C-terminal motif in mediating correct posttranslational modification of the protein. This result may have a wider implication in a novel secretory pathway in distinct members of the Cytophaga-Flavobacterium-Bacteroidetes phylum.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, University of Georgia, Life Science Bldg., Rm A322, Athens, GA 30602. Phone: (706) 542-1713. Fax: (706) 542-3719. E-mail: kyanh{at}uga.edu.

{triangledown} Published ahead of print on 1 December 2006.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, February 2007, p. 833-843, Vol. 189, No. 3
0021-9193/07/$08.00+0     doi:10.1128/JB.01530-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Monteiro, A. C., Scovino, A., Raposo, S., Gaze, V. M., Cruz, C., Svensjo, E., Narciso, M. S., Colombo, A. P., Pesquero, J. B., Feres-Filho, E., Nguyen, K.-A., Sroka, A., Potempa, J., Scharfstein, J. (2009). Kinin Danger Signals Proteolytically Released by Gingipain Induce Fimbriae-Specific IFN-{gamma}- and IL-17-Producing T Cells in Mice Infected Intramucosally with Porphyromonas gingivalis. J. Immunol. 183: 3700-3711 [Abstract] [Full Text]  
  • Nguyen, K.-A., Zylicz, J., Szczesny, P., Sroka, A., Hunter, N., Potempa, J. (2009). Verification of a topology model of PorT as an integral outer-membrane protein in Porphyromonas gingivalis. Microbiology 155: 328-337 [Abstract] [Full Text]  
  • Potempa, M., Potempa, J., Okroj, M., Popadiak, K., Eick, S., Nguyen, K.-A., Riesbeck, K., Blom, A. M. (2008). Binding of Complement Inhibitor C4b-Binding Protein Contributes to Serum Resistance of Porphyromonas gingivalis. J. Immunol. 181: 5537-5544 [Abstract] [Full Text]  
  • Mallorqui-Fernandez, N., Manandhar, S. P., Mallorqui-Fernandez, G., Uson, I., Wawrzonek, K., Kantyka, T., Sola, M., Thogersen, I. B., Enghild, J. J., Potempa, J., Gomis-Ruth, F. X. (2008). A New Autocatalytic Activation Mechanism for Cysteine Proteases Revealed by Prevotella intermedia Interpain A. J. Biol. Chem. 283: 2871-2882 [Abstract] [Full Text]  
  • Popadiak, K., Potempa, J., Riesbeck, K., Blom, A. M. (2007). Biphasic Effect of Gingipains from Porphyromonas gingivalis on the Human Complement System. J. Immunol. 178: 7242-7250 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»