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Journal of Bacteriology, March 2007, p. 1514-1522, Vol. 189, No. 5
0021-9193/07/$08.00+0     doi:10.1128/JB.01132-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Proteomic Analysis and Identification of Streptococcus pyogenes Surface-Associated Proteins{triangledown}

Anatoly Severin,1 Elliott Nickbarg,2,{dagger} Joseph Wooters,2 Shakey A. Quazi,2 Yury V. Matsuka,1 Ellen Murphy,1 Ioannis K. Moutsatsos,2 Robert J. Zagursky,1 and Stephen B. Olmsted1*

Wyeth Vaccine Research, Pearl River, New York,1 Wyeth Research, Cambridge, Massachusetts2

Received 27 July 2006/ Accepted 21 November 2006

Streptococcus pyogenes is a gram-positive human pathogen that causes a wide spectrum of disease, placing a significant burden on public health. Bacterial surface-associated proteins play crucial roles in host-pathogen interactions and pathogenesis and are important targets for the immune system. The identification of these proteins for vaccine development is an important goal of bacterial proteomics. Here we describe a method of proteolytic digestion of surface-exposed proteins to identify surface antigens of S. pyogenes. Peptides generated by trypsin digestion were analyzed by multidimensional tandem mass spectrometry. This approach allowed the identification of 79 proteins on the bacterial surface, including 14 proteins containing cell wall-anchoring motifs, 12 lipoproteins, 9 secreted proteins, 22 membrane-associated proteins, 1 bacteriophage-associated protein, and 21 proteins commonly identified as cytoplasmic. Thirty-three of these proteins have not been previously identified as cell surface associated in S. pyogenes. Several proteins were expressed in Escherichia coli, and the purified proteins were used to generate specific mouse antisera for use in a whole-cell enzyme-linked immunosorbent assay. The immunoreactivity of specific antisera to some of these antigens confirmed their surface localization. The data reported here will provide guidance in the development of a novel vaccine to prevent infections caused by S. pyogenes.

* Corresponding author. Mailing address: Wyeth Vaccine Research, Bldg. 205/Room 225, 401 North Middletown Rd., Pearl River, NY 10965. Phone: (845) 602-7992. Fax: (845) 602-4350. E-mail: olmsteds{at}wyeth.com.

{triangledown} Published ahead of print on 1 December 2006.

{dagger} Present address: Schering Plough Research Institute, 840 Memorial Dr., Cambridge, MA 02139.

Journal of Bacteriology, March 2007, p. 1514-1522, Vol. 189, No. 5
0021-9193/07/$08.00+0     doi:10.1128/JB.01132-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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