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Journal of Bacteriology, March 2007, p. 1711-1721, Vol. 189, No. 5
0021-9193/07/$08.00+0     doi:10.1128/JB.01586-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Characterization of the Transcriptional Activity of the Cryptic Plasmid pRN1 from Sulfolobus islandicus REN1H1 and Regulation of Its Replication Operon{triangledown} ,{dagger}

Silvia Berkner and Georg Lipps*

Department of Biochemistry, University of Bayreuth, 95440 Bayreuth, Germany

Received 12 October 2006/ Accepted 7 December 2006

The plasmid pRN1 from Sulfolobus islandicus REN1H1 belongs to the crenarchaeal plasmid family pRN. The plasmids in this family encode three conserved proteins that participate in plasmid replication and copy number regulation, as suggested by biochemical characterization of the recombinant proteins. In order to deepen our understanding of the molecular biology of these plasmids, we investigated the transcriptional activity of the model plasmid pRN1. We detected five major transcripts present at about 2 to 15 copies per cell. One long transcriptional unit comprises the genes for the plasmid-copy-number control protein Orf56/CopG and the replication protein Orf904. A second transcript with a long 3'-untranslated region codes for the DNA binding protein Orf80. For both transcripts, we identified countertranscripts which could play a regulatory role. The function of the fifth transcript is unclear. For the five transcripts, we determined the start site, the transcript end, the stability, and the abundance in different growth phases. Reporter gene experiments demonstrated that the copy number control protein Orf56 represses transcription of the orf56-orf904 cotranscript in vivo.

* Corresponding author. Mailing address: Department of Biochemistry, University of Bayreuth, 95440 Bayreuth, Germany. Phone: 49-921-552433. Fax: 49-921-552432. E-mail: georg.lipps{at}uni-bayreuth.de.

{triangledown} Published ahead of print on 15 December 2006.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, March 2007, p. 1711-1721, Vol. 189, No. 5
0021-9193/07/$08.00+0     doi:10.1128/JB.01586-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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