This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Overhage, J.
Right arrow Articles by Hancock, R. E. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Overhage, J.
Right arrow Articles by Hancock, R. E. W.

 Previous Article  |  Next Article 

Journal of Bacteriology, March 2007, p. 2164-2169, Vol. 189, No. 5
0021-9193/07/$08.00+0     doi:10.1128/JB.01623-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Identification of Genes Involved in Swarming Motility Using a Pseudomonas aeruginosa PAO1 Mini-Tn5-lux Mutant Library{triangledown}

Joerg Overhage, Shawn Lewenza, Alexandra K. Marr, and Robert E. W. Hancock*

Centre for Microbial Diseases and Immunity Research, University of British Columbia, 2259 Lower Mall, Vancouver, British Columbia, Canada

Received 19 October 2006/ Accepted 29 November 2006

During a screening of a mini-Tn5-luxCDABE transposon mutant library of Pseudomonas aeruginosa PAO1 for alterations in swarming motility, 36 mutants were identified with Tn5 insertions in genes for the synthesis or function of flagellin and type IV pilus, in genes for the Xcp-related type II secretion system, and in regulatory, metabolic, chemosensory, and hypothetical genes with unknown functions. These mutants were differentially affected in swimming and twitching motility but in most cases had only a minor additional motility defect. Our data provide evidence that swarming is a more complex type of motility, since it is influenced by a large number of different genes in P. aeruginosa. Conversely, many of the swarming-negative mutants also showed an impairment in biofilm formation, indicating a strong relationship between these types of growth states.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of British Columbia, 2259 Lower Mall, Lower Mall Research Station, Vancouver, British Columbia V6T 1Z4, Canada. Phone: (604) 822-2682. Fax: (604) 827-5566. E-mail: bob{at}cmdr.ubc.ca.

{triangledown} Published ahead of print on 8 December 2006.

Journal of Bacteriology, March 2007, p. 2164-2169, Vol. 189, No. 5
0021-9193/07/$08.00+0     doi:10.1128/JB.01623-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lindhout, T., Lau, P. C. Y., Brewer, D., Lam, J. S. (2009). Truncation in the core oligosaccharide of lipopolysaccharide affects flagella-mediated motility in Pseudomonas aeruginosa PAO1 via modulation of cell surface attachment. Microbiology 155: 3449-3460 [Abstract] [Full Text]  
  • Yeung, A. T. Y., Torfs, E. C. W., Jamshidi, F., Bains, M., Wiegand, I., Hancock, R. E. W., Overhage, J. (2009). Swarming of Pseudomonas aeruginosa Is Controlled by a Broad Spectrum of Transcriptional Regulators, Including MetR. J. Bacteriol. 191: 5592-5602 [Abstract] [Full Text]  
  • Gooderham, W. J., Gellatly, S. L., Sanschagrin, F., McPhee, J. B., Bains, M., Cosseau, C., Levesque, R. C., Hancock, R. E. W. (2009). The sensor kinase PhoQ mediates virulence in Pseudomonas aeruginosa. Microbiology 155: 699-711 [Abstract] [Full Text]  
  • Stewart, C. R., Rossier, O., Cianciotto, N. P. (2009). Surface Translocation by Legionella pneumophila: a Form of Sliding Motility That Is Dependent upon Type II Protein Secretion. J. Bacteriol. 191: 1537-1546 [Abstract] [Full Text]  
  • Overhage, J., Campisano, A., Bains, M., Torfs, E. C. W., Rehm, B. H. A., Hancock, R. E. W. (2008). Human Host Defense Peptide LL-37 Prevents Bacterial Biofilm Formation. Infect. Immun. 76: 4176-4182 [Abstract] [Full Text]  
  • Gooderham, W. J., Bains, M., McPhee, J. B., Wiegand, I., Hancock, R. E. W. (2008). Induction by Cationic Antimicrobial Peptides and Involvement in Intrinsic Polymyxin and Antimicrobial Peptide Resistance, Biofilm Formation, and Swarming Motility of PsrA in Pseudomonas aeruginosa. J. Bacteriol. 190: 5624-5634 [Abstract] [Full Text]  
  • Murray, T. S., Kazmierczak, B. I. (2008). Pseudomonas aeruginosa Exhibits Sliding Motility in the Absence of Type IV Pili and Flagella. J. Bacteriol. 190: 2700-2708 [Abstract] [Full Text]  
  • Kukavica-Ibrulj, I., Bragonzi, A., Paroni, M., Winstanley, C., Sanschagrin, F., O'Toole, G. A., Levesque, R. C. (2008). In Vivo Growth of Pseudomonas aeruginosa Strains PAO1 and PA14 and the Hypervirulent Strain LESB58 in a Rat Model of Chronic Lung Infection. J. Bacteriol. 190: 2804-2813 [Abstract] [Full Text]  
  • Goldberg, J. B., Hancock, R. E. W., Parales, R. E., Loper, J., Cornelis, P. (2008). Pseudomonas 2007. J. Bacteriol. 190: 2649-2662 [Full Text]  
  • Overhage, J., Bains, M., Brazas, M. D., Hancock, R. E. W. (2008). Swarming of Pseudomonas aeruginosa Is a Complex Adaptation Leading to Increased Production of Virulence Factors and Antibiotic Resistance. J. Bacteriol. 190: 2671-2679 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»