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Journal of Bacteriology, March 2007, p. 2575-2579, Vol. 189, No. 6
0021-9193/07/$08.00+0     doi:10.1128/JB.01650-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

A Single Residue Change in Vibrio harveyi Hemolysin Results in the Loss of Phospholipase and Hemolytic Activities and Pathogenicity for Turbot (Scophthalmus maximus){triangledown}

Boguang Sun,1 Xiao-Hua Zhang,1* Xuexi Tang,1 Shushan Wang,1 Yingbin Zhong,1 Jixiang Chen,1 and Brian Austin2

Department of Marine Biology, Ocean University of China, Qingdao, China,1 School of Life Sciences, Heriot-Watt University, Edinburgh EH14 4AS, United Kingdom2

Received 25 October 2006/ Accepted 10 January 2007

Vibrio harveyi hemolysin, an important virulence determinant in fish pathogenesis, was further characterized, and the enzyme was identified as a phospholipase B by gas chromatography. Site-directed mutagenesis revealed that a specific residue, Ser153, was critical for its enzymatic activity and for its virulence in fish.

* Corresponding author. Mailing address: Department of Marine Biology, Ocean University of China, 5 Yushan Road, Qingdao 266003, People's Republic of China. Phone: 86-532-82032767. Fax: 86-532-82032266. E-mail: xhzhang{at}ouc.edu.cn.

{triangledown} Published ahead of print on 12 January 2007.

Journal of Bacteriology, March 2007, p. 2575-2579, Vol. 189, No. 6
0021-9193/07/$08.00+0     doi:10.1128/JB.01650-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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