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Journal of Bacteriology, May 2007, p. 3650-3654, Vol. 189, No. 9
0021-9193/07/$08.00+0     doi:10.1128/JB.00056-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Comparative Analysis of hmuO Function and Expression in Corynebacterium Species{triangledown}

Carey A. Kunkle and Michael P. Schmitt*

Laboratory of Respiratory and Special Pathogens, Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892

Received 11 January 2007/ Accepted 13 February 2007

We have constructed defined deletions in the hmuO gene from Corynebacterium diphtheriae and Corynebacterium ulcerans and show that the C. ulcerans hmuO mutation results in a significant reduction in hemoglobin-iron utilization, whereas in C. diphtheriae strains, deletion of hmuO caused no or only partial reduction in the utilization of heme as an iron source. We also show that expression from the C. ulcerans hmuO promoter exhibits minimal regulation by iron and heme whereas transcription from the C. diphtheriae hmuO promoter shows both significant iron repression and heme-dependent activation. These findings indicate that variability in HmuO function and expression exists among Corynebacterium species.

* Corresponding author. Mailing address: DBPAP, CBER, FDA, Bldg. 29, Room 108, 8800 Rockville Pike, Bethesda, MD 20892. Phone: (301) 435-2424. Fax: (301) 402-2776. E-mail: michael.schmitt{at}fda.hhs.gov

{triangledown} Published ahead of print on 23 February 2007.

Journal of Bacteriology, May 2007, p. 3650-3654, Vol. 189, No. 9
0021-9193/07/$08.00+0     doi:10.1128/JB.00056-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Allen, C. E., Schmitt, M. P. (2009). HtaA Is an Iron-Regulated Hemin Binding Protein Involved in the Utilization of Heme Iron in Corynebacterium diphtheriae. J. Bacteriol. 191: 2638-2648 [Abstract] [Full Text]  


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