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Journal of Bacteriology, May 2007, p. 3674-3679, Vol. 189, No. 9
0021-9193/07/$08.00+0     doi:10.1128/JB.01274-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Reconstitution of the Biochemical Activities of the AttJ Repressor and the AttK, AttL, and AttM Catabolic Enzymes of Agrobacterium tumefaciens{triangledown} ,{dagger}

Yunrong Chai,{ddagger} Ching Sung Tsai, Hongbaek Cho, and Stephen C. Winans*

Department of Microbiology, Cornell University, Ithaca, New York 14853

Received 11 August 2006/ Accepted 9 February 2007

The attKLM operon encodes a lactonase (AttM) that hydrolyzes acylhomoserine lactone autoinducers, as well as two putative dehydrogenases (AttK and AttL). Here we show that AttK, AttL, and AttM collectively covert gamma-butyrolactone to succinate. Two metabolic intermediates, gamma-hydroxybutyrate and succinic semialdehyde, inactivated the AttJ repressor in vitro and induced attKLM transcription in vivo.

* Corresponding author. Mailing address: Department of Microbiology, 361A Wing Hall, Cornell University, Ithaca, NY 14853. Phone: (607) 255-2413. Fax: (607) 255-3904. E-mail: scw2{at}cornell.edu

{triangledown} Published ahead of print on 16 February 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

{ddagger} Present address: Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138.

Journal of Bacteriology, May 2007, p. 3674-3679, Vol. 189, No. 9
0021-9193/07/$08.00+0     doi:10.1128/JB.01274-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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