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Journal of Bacteriology, January 2008, p. 135-142, Vol. 190, No. 1
0021-9193/08/$08.00+0     doi:10.1128/JB.01110-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Characterization of the Arsenate Respiratory Reductase from Shewanella sp. Strain ANA-3 ^,

Davin Malasarn,^1, Jennifer R. Keeffe,² and Dianne K. Newman^1,3,4*

Division of Biology,¹ Biochemistry and Molecular Biophysics Option,² Division of Geological and Planetary Sciences,³ Howard Hughes Medical Institute, California Institute of Technology, 1200 E. California Blvd, Mail Code 100-23, Pasadena, California 91125⁴

Received 13 July 2007/ Accepted 3 September 2007

Microbial arsenate respiration contributes to the mobilization of arsenic from the solid to the soluble phase in various locales worldwide. To begin to predict the extent to which As(V) respiration impacts arsenic geochemical cycling, we characterized the expression and activity of the Shewanella sp. strain ANA-3 arsenate respiratory reductase (ARR), the key enzyme involved in this metabolism. ARR is expressed at the beginning of the exponential phase and persists throughout the stationary phase, at which point it is released from the cell. In intact cells, the enzyme localizes to the periplasm. To purify ARR, a heterologous expression system was developed in Escherichia coli. ARR requires anaerobic conditions and molybdenum for activity. ARR is a heterodimer of 131 kDa, composed of one ArrA subunit (95 kDa) and one ArrB subunit (27 kDa). For ARR to be functional, the two subunits must be expressed together. Elemental analysis of pure protein indicates that one Mo atom, four S atoms associated with a bis-molybdopterin guanine dinucleotide cofactor, and four to five [4Fe-4S] are present per ARR. ARR has an apparent melting temperature of 41°C, a K_m of 5 µM, and a V_max of 11,111 µmol of As(V) reduced min^–1 mg of protein^–1 and shows no activity in the presence of alternative electron acceptors such as antimonite, nitrate, selenate, and sulfate. The development of a heterologous overexpression system for ARR will facilitate future structural and/or functional studies of this protein family.

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* Corresponding author. Mailing address: Massachusetts Institute of Technology, Department of Biology, 77 Massachusetts Avenue, 68-380, Cambridge, MA 02139. Phone: (617) 324-2770. Fax: (617) 324-3972. E-mail: dkn{at}mit.edu

Published ahead of print on 19 October 2007.

Supplemental material for this article may be found at http://jb.asm.org/.

Present address: Department of Chemistry and Biochemistry, University of California, Los Angeles, 607 Charles E. Young Drive East, Los Angeles, CA 90095.

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Journal of Bacteriology, January 2008, p. 135-142, Vol. 190, No. 1
0021-9193/08/$08.00+0     doi:10.1128/JB.01110-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

This article has been cited by other articles:

• Murphy, J. N., Saltikov, C. W. (2009). The ArsR Repressor Mediates Arsenite-Dependent Regulation of Arsenate Respiration and Detoxification Operons of Shewanella sp. Strain ANA-3. J. Bacteriol. 191: 6722-6731 [Abstract] [Full Text]  
• Murphy, J. N., Durbin, K. J., Saltikov, C. W. (2009). Functional Roles of arcA, etrA, Cyclic AMP (cAMP)-cAMP Receptor Protein, and cya in the Arsenate Respiration Pathway in Shewanella sp. Strain ANA-3. J. Bacteriol. 191: 1035-1043 [Abstract] [Full Text]