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Journal of Bacteriology, January 2008, p. 231-239, Vol. 190, No. 1
0021-9193/08/$08.00+0     doi:10.1128/JB.00924-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Lipidation of an FlrC-Dependent Protein Is Required for Enhanced Intestinal Colonization by Vibrio cholerae{triangledown}

David C. Morris, Fen Peng, Jeffrey R. Barker, and Karl E. Klose*

South Texas Center for Emerging Infectious Diseases and Department of Biology, University of Texas San Antonio, San Antonio, Texas 78249

Received 12 June 2007/ Accepted 15 October 2007

Vibrio cholerae, the causative agent of cholera, has a sheathed, polar flagellum, and motility has been linked to virulence. An operon with two genes, flgO and flgP (VC2207 and VC2206), is positively regulated by FlrC, the activator of class III flagellar genes. Deletion of flgP results in a nonmotile phenotype, demonstrating the requirement of this gene for V. cholerae motility. V. cholerae {Delta}flgP cells synthesize fragile and defective flagella but transcribe flagellar genes similar to the wild-type strain. PhoA fusion analysis indicated that the putative lipoprotein FlgP is localized external to the cytoplasm, and fractionation demonstrated that it was localized to the outer membrane. Mutagenesis of the site of lipidation of FlgP (C18G) prevented [3H]palmitate incorporation and outer membrane localization. Interestingly, FlgP with the mutation C18G [FlgP(C18G)] could complement the {Delta}flgP mutant for motility, and the cells synthesized wild-type flagella. The {Delta}flgP mutant strain was defective for intestinal colonization (~20-fold), but FlgP(C18G) was unable to complement this defect, demonstrating that lipidation of FlgP is essential for its role in intestinal colonization but not flagellar synthesis. FlgP thus represents a novel V. cholerae intestinal colonization factor that is regulated by the flagellar transcription hierarchy.

* Corresponding author. Mailing address: Department of Biology, University of Texas at San Antonio, One UTSA Circle, San Antonio, TX 78249. Phone: (210) 458-6140. Fax: (210) 458-4468. E-mail: kklose{at}utsa.edu

{triangledown} Published ahead of print on 2 November 2007.

Journal of Bacteriology, January 2008, p. 231-239, Vol. 190, No. 1
0021-9193/08/$08.00+0     doi:10.1128/JB.00924-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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