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Journal of Bacteriology, January 2008, p. 438-441, Vol. 190, No. 1
0021-9193/08/$08.00+0     doi:10.1128/JB.01373-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Dam Methyltransferase Is Required for Stable Lysogeny of the Shiga Toxin (Stx2)-Encoding Bacteriophage 933W of Enterohemorrhagic Escherichia coli O157:H7{triangledown}

Kenan C. Murphy,1 Jennifer M. Ritchie,2 Matthew K. Waldor,2 Anders Løbner-Olesen,3 and M. G. Marinus4*

Molecular Genetics and Microbiology,1 Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605,4 The Channing Laboratory, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115,2 Department of Science, Systems and Models, Roskilde University, DK-4000 Roskilde, Denmark3

Received 23 August 2007/ Accepted 18 October 2007

Shiga toxin 2 (Stx2), one of the principal virulence factors of enterohemorrhagic Escherichia coli, is encoded by 933W, a lambda-like prophage. 933W prophage induction contributes to Stx2 production, and here, we provide evidence that Dam methyltransferase is essential for maintenance of 933W lysogeny. Our findings are consistent with the idea that the 933W prophage has a relatively low threshold for induction, which may promote Stx2 production during infection.

* Corresponding author. Mailing address: Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, MA 01605. Phone: (508) 856-3330. Fax: (508) 856-3036. E-mail: martin.marinus{at}umassmed.edu

{triangledown} Published ahead of print on 2 November 2007.

Journal of Bacteriology, January 2008, p. 438-441, Vol. 190, No. 1
0021-9193/08/$08.00+0     doi:10.1128/JB.01373-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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