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Journal of Bacteriology, January 2008, p. 447-451, Vol. 190, No. 1
0021-9193/08/$08.00+0     doi:10.1128/JB.01174-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Mapping the Transcription Start Points of the Staphylococcus aureus eap, emp, and vwb Promoters Reveals a Conserved Octanucleotide Sequence That Is Essential for Expression of These Genes{triangledown} ,{dagger}

Niamh Harraghy,1* Dagmar Homerova,2 Mathias Herrmann,1 and Jan Kormanec2

Institute of Medical Microbiology and Hygiene, University of Saarland, 66421 Homburg/Saar, Germany,1 Institute of Molecular Biology, Slovak Academy of Sciences, 84551 Bratislava, Slovakia2

Received 25 July 2007/ Accepted 19 October 2007

Mapping the transcription start points of the eap, emp, and vwb promoters revealed a conserved octanucleotide sequence (COS). Deleting this sequence abolished the expression of eap, emp, and vwb. However, electrophoretic mobility shift assays gave no evidence that this sequence was a binding site for SarA or SaeR, known regulators of eap and emp.


* Corresponding author. Present address: Institute of Biotechnology UNIL, EPFL-FSB LBTM (Bâtiment CH), Station 6, CH-1015 Lausanne, Switzerland. Phone: 41 21 693 76 16. Fax: 41 21 693 76 10. E-mail: niamh.harraghy{at}unil.ch

{triangledown} Published ahead of print on 26 October 2007.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.


Journal of Bacteriology, January 2008, p. 447-451, Vol. 190, No. 1
0021-9193/08/$08.00+0     doi:10.1128/JB.01174-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.