This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ohnishi, Y.
Right arrow Articles by Horinouchi, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ohnishi, Y.
Right arrow Articles by Horinouchi, S.

 Previous Article  |  Next Article 

Journal of Bacteriology, June 2008, p. 4050-4060, Vol. 190, No. 11
0021-9193/08/$08.00+0     doi:10.1128/JB.00204-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Genome Sequence of the Streptomycin-Producing Microorganism Streptomyces griseus IFO 13350{triangledown} ,{dagger}

Yasuo Ohnishi,1 Jun Ishikawa,2 Hirofumi Hara,1 Hirokazu Suzuki,1 Miwa Ikenoya,1 Haruo Ikeda,3 Atsushi Yamashita,3 Masahira Hattori,4* and Sueharu Horinouchi1*

Department of Biotechnology, Graduate School of Agriculture and Life Sciences, the University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan,1 Department of Bioactive Molecules, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan,2 Kitasato Institute for Life Sciences, Kitasato University, Sagamihara-shi, Kanagawa 228-8555, Japan,3 Department of Computational Biology, Graduate School of Frontier Sciences, the University of Tokyo, Kashiwa-shi, Chiba 277-8561, Japan4

Received 9 February 2008/ Accepted 19 March 2008

We determined the complete genome sequence of Streptomyces griseus IFO 13350, a soil bacterium producing an antituberculosis agent, streptomycin, which is the first aminoglycoside antibiotic, discovered more than 60 years ago. The linear chromosome consists of 8,545,929 base pairs (bp), with an average G+C content of 72.2%, predicting 7,138 open reading frames, six rRNA operons (16S-23S-5S), and 66 tRNA genes. It contains extremely long terminal inverted repeats (TIRs) of 132,910 bp each. The telomere's nucleotide sequence and secondary structure, consisting of several palindromes with a loop sequence of 5'-GGA-3', are different from those of typical telomeres conserved among other Streptomyces species. In accordance with the difference, the chromosome has pseudogenes for a conserved terminal protein (Tpg) and a telomere-associated protein (Tap), and a novel pair of Tpg and Tap proteins is instead encoded by the TIRs. Comparisons with the genomes of two related species, Streptomyces coelicolor A3(2) and Streptomyces avermitilis, clarified not only the characteristics of the S. griseus genome but also the existence of 24 Streptomyces-specific proteins. The S. griseus genome contains 34 gene clusters or genes for the biosynthesis of known or unknown secondary metabolites. Transcriptome analysis using a DNA microarray showed that at least four of these clusters, in addition to the streptomycin biosynthesis gene cluster, were activated directly or indirectly by AdpA, which is a central transcriptional activator for secondary metabolism and morphogenesis in the A-factor (a {gamma}-butyrolactone signaling molecule) regulatory cascade in S. griseus.

* Corresponding author. Mailing address for Sueharu Horinouchi: Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan. Phone: 81-3-5841-5123. Fax: 81-3-5841-8021. E-mail: asuhori{at}mail.ecc.u-tokyo.ac.jp. Mailing address for Masahira Hattori: Department of Computational Biology, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa-shi, Chiba 277-8561, Japan. Phone: 81-4-7136-4070. Fax: 81-4-7136-4084. E-mail: hattori{at}k.u-tokyo.ac.jp

{triangledown} Published ahead of print on 28 March 2008.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, June 2008, p. 4050-4060, Vol. 190, No. 11
0021-9193/08/$08.00+0     doi:10.1128/JB.00204-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Marushima, K., Ohnishi, Y., Horinouchi, S. (2009). CebR as a Master Regulator for Cellulose/Cellooligosaccharide Catabolism Affects Morphological Development in Streptomyces griseus. J. Bacteriol. 191: 5930-5940 [Abstract] [Full Text]  
  • Machado, C. X., Pinto, P. M., Zaha, A., Ferreira, H. B. (2009). A peroxiredoxin from Mycoplasma hyopneumoniae with a possible role in H2O2 detoxification. Microbiology 155: 3411-3419 [Abstract] [Full Text]  
  • Birko, Z., Swiatek, M., Szajli, E., Medzihradszky, K. F., Vijgenboom, E., Penyige, A., Keseru, J., van Wezel, G. P., Biro, S. (2009). Lack of A-factor Production Induces the Expression of Nutrient Scavenging and Stress-related Proteins in Streptomyces griseus. Mol. Cell. Proteomics 8: 2396-2403 [Abstract] [Full Text]  
  • Vecchione, J. J., Sello, J. K. (2009). A Novel Tryptophanyl-tRNA Synthetase Gene Confers High-Level Resistance to Indolmycin. Antimicrob. Agents Chemother. 53: 3972-3980 [Abstract] [Full Text]  
  • Seipke, R. F., Loria, R. (2009). Hopanoids Are Not Essential for Growth of Streptomyces scabies 87-22. J. Bacteriol. 191: 5216-5223 [Abstract] [Full Text]  
  • Tezuka, T., Hara, H., Ohnishi, Y., Horinouchi, S. (2009). Identification and Gene Disruption of Small Noncoding RNAs in Streptomyces griseus. J. Bacteriol. 191: 4896-4904 [Abstract] [Full Text]  
  • Healy, F. G., Eaton, K. P., Limsirichai, P., Aldrich, J. F., Plowman, A. K., King, R. R. (2009). Characterization of {gamma}-Butyrolactone Autoregulatory Signaling Gene Homologs in the Angucyclinone Polyketide WS5995B Producer Streptomyces acidiscabies. J. Bacteriol. 191: 4786-4797 [Abstract] [Full Text]  
  • Rezzonico, F., Stockwell, V. O., Duffy, B. (2009). Plant Agricultural Streptomycin Formulations Do Not Carry Antibiotic Resistance Genes. Antimicrob. Agents Chemother. 53: 3173-3177 [Abstract] [Full Text]  
  • Hara, H., Ohnishi, Y., Horinouchi, S. (2009). DNA microarray analysis of global gene regulation by A-factor in Streptomyces griseus. Microbiology 155: 2197-2210 [Abstract] [Full Text]  
  • Zhang, Y., Bai, L., Deng, Z. (2009). Functional characterization of the first two actinomycete 4-amino-4-deoxychorismate lyase genes. Microbiology 155: 2450-2459 [Abstract] [Full Text]  
  • Parashar, A., Colvin, K. R., Bignell, D. R. D., Leskiw, B. K. (2009). BldG and SCO3548 Interact Antagonistically To Control Key Developmental Processes in Streptomyces coelicolor. J. Bacteriol. 191: 2541-2550 [Abstract] [Full Text]  
  • Takano, H., Tanaka, T., Beppu, T., Ueda, K. (2008). Identification of sas, a conserved gene cluster involved in the regulation of aerial mycelium formation in Streptomyces griseus. Microbiology 154: 3668-3675 [Abstract] [Full Text]  
  • Bursy, J., Kuhlmann, A. U., Pittelkow, M., Hartmann, H., Jebbar, M., Pierik, A. J., Bremer, E. (2008). Synthesis and Uptake of the Compatible Solutes Ectoine and 5-Hydroxyectoine by Streptomyces coelicolor A3(2) in Response to Salt and Heat Stresses. Appl. Environ. Microbiol. 74: 7286-7296 [Abstract] [Full Text]  
  • Swiercz, J. P., Hindra, , Bobek, J., Haiser, H. J., Di Berardo, C., Tjaden, B., Elliot, M. A. (2008). Small non-coding RNAs in Streptomyces coelicolor. Nucleic Acids Res 36: 7240-7251 [Abstract] [Full Text]  
  • Di Berardo, C., Capstick, D. S., Bibb, M. J., Findlay, K. C., Buttner, M. J., Elliot, M. A. (2008). Function and Redundancy of the Chaplin Cell Surface Proteins in Aerial Hypha Formation, Rodlet Assembly, and Viability in Streptomyces coelicolor. J. Bacteriol. 190: 5879-5889 [Abstract] [Full Text]  
  • Komatsu, M., Tsuda, M., Omura, S., Oikawa, H., Ikeda, H. (2008). Identification and functional analysis of genes controlling biosynthesis of 2-methylisoborneol. Proc. Natl. Acad. Sci. USA 105: 7422-7427 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»