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Journal of Bacteriology, June 2008, p. 4088-4090, Vol. 190, No. 11
0021-9193/08/$08.00+0     doi:10.1128/JB.00016-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Identification of a Novel Mycobacterial 3-Hydroxyacyl-Thioester Dehydratase, HtdZ (Rv0130), by Functional Complementation in Yeast {triangledown}

Aner Gurvitz,1,2* J. Kalervo Hiltunen,2 and Alexander J. Kastaniotis2

Section of Physiology of Lipid Metabolism, Institute of Physiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Vienna, Austria,1 Biocenter Oulu, Department of Biochemistry, University of Oulu, Oulu, Finland2

Received 4 January 2008/ Accepted 12 March 2008

We report on the identification of Mycobacterium tuberculosis HtdZ (Rv0130), representing a novel 3-hydroxyacyl-thioester dehydratase. HtdZ was picked up by the functional complementation of Saccharomyces cerevisiae htd2{Delta} cells lacking the dehydratase of mitochondrial type II fatty acid synthase. Mutant cells expressing HtdZ contained dehydratase activity, recovered their respiratory ability, and partially restored de novo lipoic acid synthesis.

* Corresponding author. Mailing address: Medizinische Universität Wien, Zentrum für Physiologie und Pathophysiologie, Institut für Physiologie, Schwarzspanierstraße 17, 1090 Vienna, Austria. Phone: 43 1 4277 62126. Fax: 43 1 4277 62198. E-mail: aner.gurvitz{at}meduniwien.ac.at

{triangledown} Published ahead of print on 28 March 2008.

Journal of Bacteriology, June 2008, p. 4088-4090, Vol. 190, No. 11
0021-9193/08/$08.00+0     doi:10.1128/JB.00016-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gurvitz, A., Hiltunen, J. K., Kastaniotis, A. J. (2009). Heterologous Expression of Mycobacterial Proteins in Saccharomyces cerevisiae Reveals Two Physiologically Functional 3-Hydroxyacyl-Thioester Dehydratases, HtdX and HtdY, in Addition to HadABC and HtdZ. J. Bacteriol. 191: 2683-2690 [Abstract] [Full Text]  
  • Gurvitz, A., Hiltunen, J. K., Kastaniotis, A. J. (2008). Function of Heterologous Mycobacterium tuberculosis InhA, a Type 2 Fatty Acid Synthase Enzyme Involved in Extending C20 Fatty Acids to C60-to-C90 Mycolic Acids, during De Novo Lipoic Acid Synthesis in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 74: 5078-5085 [Abstract] [Full Text]  


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