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Journal of Bacteriology, June 2008, p. 4351-4359, Vol. 190, No. 12
0021-9193/08/$08.00+0     doi:10.1128/JB.00295-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

The ArgP Protein Stimulates the Klebsiella pneumoniae gdhA Promoter in a Lysine-Sensitive Manner{triangledown}

Thomas J. Goss*

Department of Molecular, Cellular and Developmental Biology, The University of Michigan, Ann Arbor, Michigan 48109-1048

Received 27 February 2008/ Accepted 5 April 2008

The lysine-sensitive factor that binds to the upstream region of the Klebsiella pneumoniae gdhA promoter and stimulates gdhA transcription during growth in minimal medium has been proposed to be the K. pneumoniae ArgP protein (M. R. Nandineni, R. S. Laishram, and J. Gowrishankar, J. Bacteriol. 186:6391-6399, 2004). A knockout mutation of the K. pneumoniae argP gene was generated and used to assess the roles of exogenous lysine and argP in the regulation of the gdhA promoter. Disruption of argP reduced the strength and the lysine-dependent regulation of the gdhA promoter. Electrophoretic mobility shift assays using crude extracts prepared from wild-type and argP-defective strains indicted the presence of an argP-dependent factor whose ability to bind the gdhA promoter was lysine sensitive. DNase I footprinting studies using purified K. pneumoniae ArgP protein indicated that ArgP bound the region that lies approximately 50 to 100 base pairs upstream of the gdhA transcription start site in a manner that was sensitive to the presence of lysine. Substitutions within the region bound by ArgP affected the binding of ArgP to the gdhA promoter region in vitro and the argP-dependent stimulation of the gdhA promoter in vivo. These observations suggest that elevated intracellular levels of lysine reduce the affinity of ArgP for its binding site at the gdhA promoter, preventing ArgP from binding to and stimulating transcription from the promoter in vivo.

* Mailing address: Department of Molecular, Cellular and Developmental Biology, the University of Michigan, Ann Arbor, MI 48109-1048. Phone: (734) 763-4681. Fax: (734) 647-0884. E-mail: taaj{at}umich.edu

{triangledown} Published ahead of print on 18 April 2008.

Journal of Bacteriology, June 2008, p. 4351-4359, Vol. 190, No. 12
0021-9193/08/$08.00+0     doi:10.1128/JB.00295-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bouvier, J., Stragier, P., Morales, V., Remy, E., Gutierrez, C. (2008). Lysine Represses Transcription of the Escherichia coli dapB Gene by Preventing Its Activation by the ArgP Activator. J. Bacteriol. 190: 5224-5229 [Abstract] [Full Text]  


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