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Journal of Bacteriology, July 2008, p. 4596-4602, Vol. 190, No. 13
0021-9193/08/$08.00+0     doi:10.1128/JB.00262-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Salmonella enterica Requires ApbC Function for Growth on Tricarballylate: Evidence of Functional Redundancy between ApbC and IscU{triangledown}

Jeffrey M. Boyd, Jeffrey A. Lewis, Jorge C. Escalante-Semerena, and Diana M. Downs*

Department of Bacteriology, University of Wisconsin, Madison, Madison, Wisconsin 53706

Received 20 February 2008/ Accepted 13 April 2008

Mutants of Salmonella enterica lacking apbC have nutritional and biochemical properties indicative of defects in [Fe-S] cluster metabolism. Here we show that apbC is required for S. enterica to use tricarballylate as a carbon and energy source. Tricarballylate catabolism requires three gene products, TcuA, TcuB, and TcuC. Of relevance to this work is the TcuB protein, which has two [4Fe-4S] clusters required for function, making it a logical target for the apbC effect. TcuB activity was 100-fold lower in an apbC mutant than in the isogenic apbC+ strain. Genetic data show that derepression of the iscRSUA-hscAB-fdx-orf3 operon or overexpression of iscU from a plasmid compensates for the lack of ApbC during growth on tricarballylate. The studies described herein provide evidence that the scaffold protein IscU has a functional overlap with ApbC and that ApbC function is involved in the synthesis of active TcuB.

* Corresponding author. Mailing address: Department of Bacteriology, 1550 Linden Drive, 6472 Microbial Sciences Building, University of Wisconsin, Madison, WI 53706. Phone: (608) 265-4630. Fax: (608) 890-0785. E-mail: downs{at}bact.wisc.edu

{triangledown} Published ahead of print on 25 April 2008.

Journal of Bacteriology, July 2008, p. 4596-4602, Vol. 190, No. 13
0021-9193/08/$08.00+0     doi:10.1128/JB.00262-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lewis, J. A., Boyd, J. M., Downs, D. M., Escalante-Semerena, J. C. (2009). Involvement of the Cra Global Regulatory Protein in the Expression of the iscRSUA Operon, Revealed during Studies of Tricarballylate Catabolism in Salmonella enterica. J. Bacteriol. 191: 2069-2076 [Abstract] [Full Text]  
  • Boyd, J. M., Drevland, R. M., Downs, D. M., Graham, D. E. (2009). Archaeal ApbC/Nbp35 Homologs Function as Iron-Sulfur Cluster Carrier Proteins. J. Bacteriol. 191: 1490-1497 [Abstract] [Full Text]  
  • Boyd, J. M., Sondelski, J. L., Downs, D. M. (2009). Bacterial ApbC Protein Has Two Biochemical Activities That Are Required for in Vivo Function. J. Biol. Chem. 284: 110-118 [Abstract] [Full Text]  


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