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Journal of Bacteriology, July 2008, p. 4764-4771, Vol. 190, No. 13
0021-9193/08/$08.00+0     doi:10.1128/JB.02021-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Functional Analysis of the Essential GTP-Binding-Protein-Coding Gene cgtA of Vibrio cholerae{triangledown}

Sangita Shah, Bhabatosh Das, and Rupak K. Bhadra*

Infectious Diseases & Immunology Division, Indian Institute of Chemical Biology, Kolkata 700032, India

Received 31 December 2007/ Accepted 22 April 2008

The cgtA gene, coding for the conserved G protein CgtA, is essential in bacteria. In contrast to a previous report, here we show by using genetic analysis that cgtA is essential in Vibrio cholerae even in a {Delta}relA background. Depletion of CgtA affected the growth of V. cholerae and rendered the cells highly sensitive to the replication inhibitor hydroxyurea. Overexpression of V. cholerae CgtA caused distinct elongation of Escherichia coli cells. Deletion analysis indicated that the C-terminal end of CgtA plays a critical role in its proper function.

* Corresponding author. Mailing address: Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, Jadavpur, Kolkata 700 032, India. Phone: 91-33-2473-3491. Fax: 91-33-2473-5197. E-mail: rupakbhadra{at}iicb.res.in

{triangledown} Published ahead of print on 2 May 2008.

Journal of Bacteriology, July 2008, p. 4764-4771, Vol. 190, No. 13
0021-9193/08/$08.00+0     doi:10.1128/JB.02021-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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