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Journal of Bacteriology, September 2008, p. 5944-5952, Vol. 190, No. 17
0021-9193/08/$08.00+0     doi:10.1128/JB.00763-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Salmonella enterica Serovar Typhimurium BipA Exhibits Two Distinct Ribosome Binding Modes{triangledown}

Megan A. deLivron and Victoria L. Robinson*

Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269

Received 28 May 2008/ Accepted 30 June 2008

BipA is a highly conserved prokaryotic GTPase that functions to influence numerous cellular processes in bacteria. In Escherichia coli and Salmonella enterica serovar Typhimurium, BipA has been implicated in controlling bacterial motility, modulating attachment and effacement processes, and upregulating the expression of virulence genes and is also responsible for avoidance of host defense mechanisms. In addition, BipA is thought to be involved in bacterial stress responses, such as those associated with virulence, temperature, and symbiosis. Thus, BipA is necessary for securing bacterial survival and successful invasion of the host. Steady-state kinetic analysis and pelleting assays were used to assess the GTPase and ribosome-binding properties of S. enterica BipA. Under normal bacterial growth, BipA associates with the ribosome in the GTP-bound state. However, using sucrose density gradients, we demonstrate that the association of BipA and the ribosome is altered under stress conditions in bacteria similar to those experienced during virulence. The data show that this differential binding is brought about by the presence of ppGpp, an alarmone that signals the onset of stress-related events in bacteria.

* Corresponding author. Mailing address: University of Connecticut, Dept. of Molecular and Cell Biology, 91 N. Eagleville Rd., Unit 3125, Storrs, CT 06250. Phone: (860) 486-4353. Fax: (860) 486-4331. E-mail: victoria.robinson{at}uconn.edu

{triangledown} Published ahead of print on 11 July 2008.

Journal of Bacteriology, September 2008, p. 5944-5952, Vol. 190, No. 17
0021-9193/08/$08.00+0     doi:10.1128/JB.00763-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Tomar, S. K., Dhimole, N., Chatterjee, M., Prakash, B. (2009). Distinct GDP/GTP bound states of the tandem G-domains of EngA regulate ribosome binding. Nucleic Acids Res 37: 2359-2370 [Abstract] [Full Text]  


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