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Journal of Bacteriology, September 2008, p. 6037, Vol. 190, No. 17
0021-9193/08/$08.00+0     doi:10.1128/JB.00867-08

AUTHOR'S CORRECTION

The {sigma}E Cell Envelope Stress Response of Streptomyces coelicolor Is Influenced by a Novel Lipoprotein, CseA

Matthew I. Hutchings, Hee-Jeon Hong, Emmanuelle Leibovitz, Iain C. Sutcliffe, and Mark J. Buttner

Department of Molecular Microbiology, John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom, and the Biomolecular and Biomedical Research Centre, School of Applied Science, Northumbria University, Newcastle upon Tyne NE1 8ST, United Kingdom

Volume 188, no. 20, p. 7222-7229, 2006. Page 7225, Figure 1B: The data in this figure are correct. However, the {Delta}cseA panel was previously published in error, labeled "{Delta}sigE," in reference 1, and the {Delta}sigE panel has therefore never been published. To clarify, the complete data set, originally split between the two papers, is reproduced here. A similar correction will appear in Molecular Microbiology (2).


Figure 1
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  		FIG. 1. Induction of the sigE promoter (sigEp) in its native chromosomal context by vancomycin. S1 nuclease protection analysis of the sigE transcript in S. coelicolor M600 (wt), J2130 ({Delta}sigE), J2172 ({Delta}cseA), and J2142 ({Delta}cseB::hyg) grown to mid- to late exponential phase in NMMP liquid medium containing 2 mM MgCl2 and exposed to 10 µg/ml vancomycin. RNA was extracted from samples taken immediately prior to the addition of vancomycin and at subsequent 30-min intervals. Relative signal strengths were quantified on a Phosphorimager; each signal is expressed as a percentage of the strongest signal.

References

    1
  1. Hong, H.-J., M. S. B. Paget, and M. J. Buttner. 2002. A signal transduction system in Streptomyces coelicolor that activates the expression of a putative cell wall glycan operon in response to vancomycin and other cell wall-specific antibiotics. Mol. Microbiol. 44:1199-1211.[CrossRef][Medline]
    2. 2
  2. Hong, H.-J., M. S. B. Paget, and M. J. Buttner. 2008. Corrigendum: a signal transduction system in Streptomyces coelicolor that activates expression of a putative cell wall glycan operon in response to vancomycin and other cell wall-specific antibiotics. Mol. Microbiol. 69:1069.[CrossRef][Medline]

Journal of Bacteriology, September 2008, p. 6037, Vol. 190, No. 17
0021-9193/08/$08.00+0     doi:10.1128/JB.00867-08





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