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Journal of Bacteriology, September 2008, p. 6258-6263, Vol. 190, No. 18
0021-9193/08/$08.00+0     doi:10.1128/JB.00630-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Genomic Analysis of the Role of RNase R in the Turnover of Pseudomonas putida mRNAs{triangledown} ,{dagger}

Pilar Fonseca, Renata Moreno, and Fernando Rojo*

Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, CSIC, Campus de la Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain

Received 6 May 2008/ Accepted 6 July 2008

RNase R is a 3'-5' highly processive exoribonuclease that can digest RNAs with extensive secondary structure. We analyzed the global effect of eliminating RNase R on the Pseudomonas putida transcriptome and the expression of the rnr gene under diverse conditions. The absence of RNase R led to increased levels of many mRNAs, indicating that it plays an important role in mRNA turnover.

* Corresponding author. Mailing address: Centro Nacional de Biotecnología, CSIC, Campus de la Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain. Phone: (34) 91 585 45 39. Fax: (34) 91 585 45 06. E-mail: frojo{at}cnb.csic.es

{triangledown} Published ahead of print on 18 July 2008.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, September 2008, p. 6258-6263, Vol. 190, No. 18
0021-9193/08/$08.00+0     doi:10.1128/JB.00630-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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