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Journal of Bacteriology, January 2008, p. 515-526, Vol. 190, No. 2
0021-9193/08/$08.00+0     doi:10.1128/JB.01219-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Characterization of Integrative and Conjugative Element ICEKp1-Associated Genomic Heterogeneity in a Klebsiella pneumoniae Strain Isolated from a Primary Liver Abscess{triangledown}

Tzu-Lung Lin,1 Cha-Ze Lee,2 Pei-Fang Hsieh,1 Shih-Feng Tsai,3,4 and Jin-Town Wang1,2*

Department of Microbiology, National Taiwan University College of Medicine, Taipei,1 Department of Internal Medicine, National Taiwan University Hospital, Taipei,2 Faculty of Life Sciences and Institute of Genetics, National Yang-Ming University, Taipei,3 Division of Molecular and Genomic Medicine, National Health Research Institute, Zhunan, Miaoli, Taiwan4

Received 29 July 2007/ Accepted 25 October 2007

Genomic heterogeneity has been shown to be associated with Klebsiella pneumoniae strains causing pyogenic liver abscesses (PLA) and metastatic infections. In order to explore the mechanism responsible for genomic heterogeneity in K. pneumoniae, we compared the complete genomic sequences of strains NTUH-K2044 and MGH78578. An ~76-kbp DNA fragment located adjacent to an asparagine (asn) tRNA gene was present in NTUH-K2044 but not in MGH78578. This fragment could be divided into three regions with different functions, and structurally it resembled a functional integrative and conjugative element (ICE), ICEEc1, in Escherichia coli. The 5' region of this fragment contained genes similar to a high-pathogenicity island (HPI) of Yersinia pestis and Yersinia pseudotuberculosis. The middle region was similar to part of a large plasmid in K. pneumoniae, and the 3' region contained genes responsible for DNA conjugative transfer. Therefore, this DNA fragment was designated ICEKp1. Precise excision and extrachromosomal circularization of ICEKp1 were detected in K. pneumoniae wild-type strain NTUH-K2044. ICEKp1 could integrate into the asn tRNA loci of the chromosome of another K. pneumoniae isolate. The prevalence of ICEKp1 was higher in PLA strains (38 of 42 strains) than in non-tissue-invasive strains (5 of 32 strains). Therefore, ICEKp1 may contribute to the transmission of the HPI and result in K. pneumoniae PLA infection-associated genomic heterogeneity.

* Corresponding author. Mailing address: Department of Microbiology, National Taiwan University College of Medicine, 1, Sec 1, Jen-Ai Rd., Taipei, Taiwan. Phone: 886-2-23123456, ext. 8292. Fax: 886-2-23948718. E-mail: wangjt{at}ntu.edu.tw

{triangledown} Published ahead of print on 2 November 2007.

Journal of Bacteriology, January 2008, p. 515-526, Vol. 190, No. 2
0021-9193/08/$08.00+0     doi:10.1128/JB.01219-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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