This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Thein, M.
Right arrow Articles by Benz, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Thein, M.
Right arrow Articles by Benz, R.

 Previous Article  |  Next Article 

Journal of Bacteriology, November 2008, p. 7035-7042, Vol. 190, No. 21
0021-9193/08/$08.00+0     doi:10.1128/JB.00818-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Oms38 Is the First Identified Pore-Forming Protein in the Outer Membrane of Relapsing Fever Spirochetes{triangledown}

Marcus Thein,1 Ignas Bunikis,2 Katrin Denker,3 Christer Larsson,2 Sally Cutler,4 Michel Drancourt,5 Tom G. Schwan,6 Reinhard Mentele,7 Friedrich Lottspeich,7 Sven Bergström,2 and Roland Benz1*

Department of Biotechnology, Biocenter, University of Würzburg, Würzburg, Germany,1 Department of Molecular Biology, Umeå University, Umeå, Sweden,2 Rudolf-Virchow-Center, DFG-Research Center for Experimental Biomedicine, University of Würzburg, Würzburg, Germany,3 University of East London, School of Health and Bioscience, Stratford, London, United Kingdom,4 Unité des Rickettsies, CNRS UMR 6020, IFR 48, Université de la Méditerranée, Marseille, France,5 Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840,6 Max-Planck Institute of Biochemistry, Martinsried, Germany7

Received 10 June 2008/ Accepted 18 August 2008

Relapsing fever is a worldwide, endemic disease caused by several spirochetal species belonging to the genus Borrelia. During the recurring fever peaks, borreliae proliferate remarkably quickly compared to the slow dissemination of Lyme disease Borrelia and therefore require efficient nutrient uptake from the blood of their hosts. This study describes the identification and characterization of the first relapsing fever porin, which is present in the outer membranes of B. duttonii, B. hermsii, B. recurrentis, and B. turicatae. The pore-forming protein was purified by hydroxyapatite chromatography and designated Oms38, for outer membrane-spanning protein of 38 kDa. Biophysical characterization of Oms38 was done by using the black lipid bilayer method, demonstrating that Oms38 forms small, water-filled channels of 80 pS in 1 M KCl that did not exhibit voltage-dependent closure. The Oms38 channel is slightly selective for anions and shows a ratio of permeability for cations over anions of 0.41 in KCl. Analysis of the deduced amino acid sequences demonstrated that Oms38 contains an N-terminal signal sequence which is processed under in vivo conditions. Oms38 is highly conserved within the four studied relapsing fever species, sharing an overall amino acid identity of 58% and with a strong indication for the presence of amphipathic β-sheets.

* Corresponding author. Mailing address: Department of Biotechnology, Biocenter, University of Würzburg, Am Hubland, D-97074 Würzburg, Germany. Phone: 49-(0)931-888-4501. Fax: 49-(0)931-888-4509. E-mail: roland.benz{at}mail.uni-wuerzburg.de

{triangledown} Published ahead of print on 29 August 2008.

Journal of Bacteriology, November 2008, p. 7035-7042, Vol. 190, No. 21
0021-9193/08/$08.00+0     doi:10.1128/JB.00818-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Izard, J., Renken, C., Hsieh, C.-E., Desrosiers, D. C., Dunham-Ems, S., La Vake, C., Gebhardt, L. L., Limberger, R. J., Cox, D. L., Marko, M., Radolf, J. D. (2009). Cryo-Electron Tomography Elucidates the Molecular Architecture of Treponema pallidum, the Syphilis Spirochete. J. Bacteriol. 191: 7566-7580 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»