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Journal of Bacteriology, November 2008, p. 7584-7590, Vol. 190, No. 22
0021-9193/08/$08.00+0     doi:10.1128/JB.00856-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Role of Two NtcA-Binding Sites in the Complex ntcA Gene Promoter of the Heterocyst-Forming Cyanobacterium Anabaena sp. Strain PCC 7120{triangledown} ,{dagger}

Elvira Olmedo-Verd, Ana Valladares, Enrique Flores, Antonia Herrero, and Alicia M. Muro-Pastor*

Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas and Universidad de Sevilla, E-41092 Seville, Spain

Received 23 June 2008/ Accepted 8 September 2008

Anabaena sp. strain PCC 7120 is a filamentous cyanobacterium that fixes N2 in specialized cells called heterocysts, which differentiate from vegetative cells in a process that requires the nitrogen control transcription factor NtcA. 2-Oxoglutarate-stimulated binding of purified NtcA to wild-type and modified versions of the ntcA gene promoter from Anabaena sp. was analyzed by mobility shift and DNase I footprinting assays, and the role of NtcA-binding sites in the expression of the ntcA gene during heterocyst differentiation was studied in vivo by using an ntcA-gfp translational fusion and primer extension analysis. Mutation of neither of the two identified NtcA-binding sites eliminated localized expression of ntcA in proheterocysts, but mutation of both sites led to very low, nonlocalized expression.

* Corresponding author. Mailing address: Instituto de Bioquímica Vegetal y Fotosíntesis, Centro de Investigaciones Científicas Isla de la Cartuja, Avda. Américo Vespucio 49, E-41092 Seville, Spain. Phone: 34 95 448 9521. Fax: 34 95 446 0065. E-mail: alicia{at}ibvf.csic.es

{triangledown} Published ahead of print on 19 September 2008.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, November 2008, p. 7584-7590, Vol. 190, No. 22
0021-9193/08/$08.00+0     doi:10.1128/JB.00856-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Muro-Pastor, A. M., Flores, E., Herrero, A. (2009). NtcA-Regulated Heterocyst Differentiation Genes hetC and devB from Anabaena sp. Strain PCC 7120 Exhibit a Similar Tandem Promoter Arrangement. J. Bacteriol. 191: 5765-5774 [Abstract] [Full Text]  


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