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Journal of Bacteriology, December 2008, p. 7684-7692, Vol. 190, No. 23
0021-9193/08/$08.00+0     doi:10.1128/JB.01010-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Streptomyces scabies 87-22 Possesses a Functional Tomatinase{triangledown}

Ryan F. Seipke and Rosemary Loria*

Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, New York 14853

Received 22 July 2008/ Accepted 22 September 2008

The actinomycete Streptomyces scabies 87-22 is the causal agent of common scab, an economically important disease of potato and taproot crops. Sequencing of the S. scabies 87-22 genome revealed the presence of a gene with high homology to the gene encoding the {alpha}-tomatine-detoxifying enzyme tomatinase found in fungal tomato pathogens. The tomA gene from S. scabies 87-22 was cotranscribed with a putative family 1 glycosyl hydrolase gene, and purified TomA protein was active only on {alpha}-tomatine and not potato glycoalkaloids or xylans. Tomatinase-null mutants were more sensitive to {alpha}-tomatine than the wild-type strain in a disk diffusion assay. Interestingly, tomatine affected only aerial mycelium and not vegetative mycelium, suggesting that the target(s) of {alpha}-tomatine is not present during vegetative growth. Severities of disease for tomato seedlings affected by S. scabies 87-22 wild-type and {Delta}tomA1 strains were indistinguishable, suggesting that tomatinase is not important in pathogenicity on tomato plants. However, conservation of tomA on a pathogenicity island in S. acidiscabies and S. turgidiscabies suggests a role in plant-microbe interaction.

* Corresponding author. Mailing address: Cornell University, 334 Plant Science Building, Ithaca, NY 14853. Phone: (607) 255-7831. Fax: (607) 255-4471. E-mail: rl21{at}cornell.edu

{triangledown} Published ahead of print on 3 October 2008.

Journal of Bacteriology, December 2008, p. 7684-7692, Vol. 190, No. 23
0021-9193/08/$08.00+0     doi:10.1128/JB.01010-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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