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Journal of Bacteriology, December 2008, p. 7975-7984, Vol. 190, No. 24
0021-9193/08/$08.00+0     doi:10.1128/JB.00879-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Posttranscriptional Regulation of Flagellin Synthesis in Helicobacter pylori by the RpoN Chaperone HP0958{triangledown} ,{dagger}

Francois P. Douillard,1 Kieran A. Ryan,1 Delphine L. Caly,1 Jason Hinds,2 Adam A. Witney,2 Sarah E. Husain,2 and Paul W. O'Toole1*

Department of Microbiology & Alimentary Pharmabiotic Centre, University College Cork, Ireland,1 Bacterial Microarray Group, Division of Cellular and Molecular Medicine, St. George's University of London, Cranmer Terrace, London, United Kingdom2

Received 26 June 2008/ Accepted 7 October 2008

The Helicobacter pylori protein HP0958 is essential for flagellum biogenesis. It has been shown that HP0958 stabilizes the {sigma}54 factor RpoN. The aim of this study was to further investigate the role of HP0958 in flagellum production in H. pylori. Global transcript analysis identified a number of flagellar genes that were differentially expressed in an HP0958 mutant strain. Among these, the transcription of the major flagellin gene flaA was upregulated twofold, suggesting that HP0958 was a negative regulator of the flaA gene. However, the production of the FlaA protein was significantly reduced in the HP0958 mutant, and this was not due to the decreased stability of the FlaA protein. RNA stability analysis and binding assays indicated that HP0958 binds and destabilizes flaA mRNA. The HP0958 mutant was successfully complemented, confirming that the mutant phenotype described was due to the lack of HP0958. We conclude that HP0958 is a posttranscriptional regulator that modulates the amount of the flaA message available for translation in H. pylori.

* Corresponding author. Mailing address: University College Cork, Department of Microbiology, Food Science Building, Western Road, Cork, Ireland. Phone: 353 21 490 3997. Fax: 353 21 490 3101. E-mail: pwotoole{at}ucc.ie

{triangledown} Published ahead of print on 17 October 2008.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, December 2008, p. 7975-7984, Vol. 190, No. 24
0021-9193/08/$08.00+0     doi:10.1128/JB.00879-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Douillard, F. P., Ryan, K. A., Hinds, J., O'Toole, P. W. (2009). Effect of FliK mutation on the transcriptional activity of the {sigma}54 sigma factor RpoN in Helicobacter pylori. Microbiology 155: 1901-1911 [Abstract] [Full Text]  


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