This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Moeller, R.
Right arrow Articles by Nicholson, W. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Moeller, R.
Right arrow Articles by Nicholson, W. L.

 Previous Article  |  Next Article 

Journal of Bacteriology, February 2008, p. 1134-1140, Vol. 190, No. 3
0021-9193/08/$08.00+0     doi:10.1128/JB.01644-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Roles of the Major, Small, Acid-Soluble Spore Proteins and Spore-Specific and Universal DNA Repair Mechanisms in Resistance of Bacillus subtilis Spores to Ionizing Radiation from X Rays and High-Energy Charged-Particle Bombardment{triangledown}

Ralf Moeller,1* Peter Setlow,2 Gerda Horneck,1 Thomas Berger,1 Günther Reitz,1 Petra Rettberg,1 Aidan J. Doherty,3 Ryuichi Okayasu,4 and Wayne L. Nicholson5

German Aerospace Center, Institute of Aerospace Medicine, Radiation Biology Division, Cologne, Germany,1 Department of Molecular, Microbial and Structural Biology, University of Connecticut Health Center, Farmington, Connecticut,2 Genome Damage and Stability Centre, University of Sussex, Brighton BN1, United Kingdom,3 International Space Radiation Laboratory, National Institute of Radiological Sciences, Chiba-shi, Japan,4 University of Florida, Department of Microbiology and Cell Science, Space Life Sciences Laboratory, Kennedy Space Center, Florida5

Received 11 October 2007/ Accepted 15 November 2007

The role of DNA repair by nonhomologous end joining (NHEJ), homologous recombination, spore photoproduct lyase, and DNA polymerase I and genome protection via {alpha}/β-type small, acid-soluble spore proteins (SASP) in Bacillus subtilis spore resistance to accelerated heavy ions (high-energy charged [HZE] particles) and X rays has been studied. Spores deficient in NHEJ and {alpha}/β-type SASP were significantly more sensitive to HZE particle bombardment and X-ray irradiation than were the recA, polA, and splB mutant and wild-type spores, indicating that NHEJ provides an efficient DNA double-strand break repair pathway during spore germination and that the loss of the {alpha}/β-type SASP leads to a significant radiosensitivity to ionizing radiation, suggesting the essential function of these spore proteins as protectants of spore DNA against ionizing radiation.

* Corresponding author. Mailing address: German Aerospace Center (DLR), Institute of Aerospace Medicine, Radiation Biology Division, Department of Photo- and Exobiology, Linder Hoehe, D-51147 Cologne, Germany. Phone: 49(2203) 601-3145. Fax: 49(2203) 61790. E-mail: ralf.moeller{at}dlr.de

{triangledown} Published ahead of print on 30 November 2007.

Journal of Bacteriology, February 2008, p. 1134-1140, Vol. 190, No. 3
0021-9193/08/$08.00+0     doi:10.1128/JB.01644-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Moeller, R., Setlow, P., Reitz, G., Nicholson, W. L. (2009). Roles of Small, Acid-Soluble Spore Proteins and Core Water Content in Survival of Bacillus subtilis Spores Exposed to Environmental Solar UV Radiation. Appl. Environ. Microbiol. 75: 5202-5208 [Abstract] [Full Text]  
  • Simmons, L. A., Goranov, A. I., Kobayashi, H., Davies, B. W., Yuan, D. S., Grossman, A. D., Walker, G. C. (2009). Comparison of Responses to Double-Strand Breaks between Escherichia coli and Bacillus subtilis Reveals Different Requirements for SOS Induction. J. Bacteriol. 191: 1152-1161 [Abstract] [Full Text]  
  • Moeller, R., Horneck, G., Rabbow, E., Reitz, G., Meyer, C., Hornemann, U., Stoffler, D. (2008). Role of DNA Protection and Repair in Resistance of Bacillus subtilis Spores to Ultrahigh Shock Pressures Simulating Hypervelocity Impacts. Appl. Environ. Microbiol. 74: 6682-6689 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»