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Journal of Bacteriology, February 2008, p. 915-925, Vol. 190, No. 3
0021-9193/08/$08.00+0     doi:10.1128/JB.01647-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

The Campylobacter jejuni NADH:Ubiquinone Oxidoreductase (Complex I) Utilizes Flavodoxin Rather than NADH{triangledown}

Dilan R. Weerakoon and Jonathan W. Olson*

Department of Microbiology, North Carolina State University, Raleigh, North Carolina

Received 11 October 2007/ Accepted 18 November 2007

Campylobacter jejuni encodes 12 of the 14 subunits that make up the respiratory enzyme NADH:ubiquinone oxidoreductase (also called complex I). The two nuo genes not present in C. jejuni encode the NADH dehydrogenase, and in their place in the operon are the novel genes designated Cj1575c and Cj1574c. A series of mutants was generated in which each of the 12 nuo genes (homologues to known complex I subunits) was disrupted or deleted. Each of the nuo mutants will not grow in amino acid-based medium unless supplemented with an alternative respiratory substrate such as formate. Unlike the nuo genes, Cj1574c is an essential gene and could not be disrupted unless an intact copy of the gene was provided at an unrelated site on the chromosome. A nuo deletion mutant can efficiently respire formate but is deficient in {alpha}-ketoglutarate respiratory activity compared to the wild type. In C. jejuni, {alpha}-ketoglutarate respiration is mediated by the enzyme 2-oxoglutarate:acceptor oxidoreductase; mutagenesis of this enzyme abolishes {alpha}-ketoglutarate-dependent O2 uptake and fails to reduce the electron transport chain. The electron acceptor for 2-oxoglutarate:acceptor oxidoreductase was determined to be flavodoxin, which was also determined to be an essential protein in C. jejuni. A model is presented in which CJ1574 mediates electron flow into the respiratory transport chain from reduced flavodoxin and through complex I.

* Corresponding author. Mailing address: Department of Microbiology, Campus Box 7615, North Carolina State University, Raleigh, NC 27695. Phone: (919) 515-7860. Fax: (919) 515-7867. E-mail: jwolson{at}ncsu.edu

{triangledown} Published ahead of print on 7 December 2007.

Journal of Bacteriology, February 2008, p. 915-925, Vol. 190, No. 3
0021-9193/08/$08.00+0     doi:10.1128/JB.01647-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Vegge, C. S., Brondsted, L., Li, Y.-P., Bang, D. D., Ingmer, H. (2009). Energy Taxis Drives Campylobacter jejuni toward the Most Favorable Conditions for Growth. Appl. Environ. Microbiol. 75: 5308-5314 [Abstract] [Full Text]  
  • Wright, J. A., Grant, A. J., Hurd, D., Harrison, M., Guccione, E. J., Kelly, D. J., Maskell, D. J. (2009). Metabolite and transcriptome analysis of Campylobacter jejuni in vitro growth reveals a stationary-phase physiological switch. Microbiology 155: 80-94 [Abstract] [Full Text]  


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