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Journal of Bacteriology, March 2008, p. 1499-1506, Vol. 190, No. 5
0021-9193/08/$08.00+0     doi:10.1128/JB.01160-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Transcriptional Regulation of the Clostridium cellulolyticum cip-cel Operon: a Complex Mechanism Involving a Catabolite-Responsive Element{triangledown}

Laetitia Abdou,1,2 Céline Boileau,1 Pascale de Philip,1,2 Sandrine Pagès,1,2 Henri-Pierre Fiérobe,1 and Chantal Tardif1,2*

Laboratoire de Bioénergétique et Ingénierie des Protéines, UPR 9036-CNRS,1 Université d'Aix-Marseille, Marseille, France2

Received 23 July 2007/ Accepted 10 December 2007

The cip-cel cluster of genes plays an important role in the catabolism of the substrate cellulose by Clostridium cellulolyticum. It encodes several key components of the cellulosomes, including the scaffolding protein CipC and the major cellulase Cel48F. All the genes of this cluster display linked transcription, focusing attention on the promoter upstream from the first gene, cipC. We analyzed the regulation of the cipC promoter using a transcriptional fusion approach. A single promoter is located between nucleotides –671 and –643 with respect to the ATG start codon, and the large mRNA leader sequence is processed at position –194. A catabolite-responsive element (CRE) 414 nucleotides downstream from the transcriptional start site has been shown to be involved in regulating this operon by a carbon catabolite repression mechanism. This CRE is thought to bind a CcpA-like regulator complexed with a P-Ser-Crh-like protein. Sequences surrounding the promoter sequence may also be involved in direct (sequence-dependent DNA curvature) or indirect (unknown regulator binding) regulation.

* Corresponding author. Mailing address: BIP-CNRS, 31 chemin Joseph Aiguier, 13402 Marseille Cedex 20, France. Phone: (33) 4 91 16 43 40. Fax: (33) 4 91 71 33 21. E-mail: tardif{at}ibsm.cnrs-mrs.fr

{triangledown} Published ahead of print on 21 December 2007.

Journal of Bacteriology, March 2008, p. 1499-1506, Vol. 190, No. 5
0021-9193/08/$08.00+0     doi:10.1128/JB.01160-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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