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Journal of Bacteriology, March 2008, p. 1699-1709, Vol. 190, No. 5
0021-9193/08/$08.00+0     doi:10.1128/JB.01176-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Large-Scale Transposon Mutagenesis of Photobacterium profundum SS9 Reveals New Genetic Loci Important for Growth at Low Temperature and High Pressure{triangledown}

Federico M. Lauro,1 Khiem Tran,1 Alessandro Vezzi,2 Nicola Vitulo,2 Giorgio Valle,2 and Douglas H. Bartlett1*

Center for Marine Biotechnology and Biomedicine, Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, California 92093-0202,1 G.R.U.P.-CRIBI Biotechnology Centre, University of Padua, via U. Bassi, 58/b, 35131, Padova, Italy2

Received 25 July 2007/ Accepted 10 December 2007

Microorganisms adapted to piezopsychrophilic growth dominate the majority of the biosphere that is at relatively constant low temperatures and high pressures, but the genetic bases for the adaptations are largely unknown. Here we report the use of transposon mutagenesis with the deep-sea bacterium Photobacterium profundum strain SS9 to isolate dozens of mutant strains whose growth is impaired at low temperature and/or whose growth is altered as a function of hydrostatic pressure. In many cases the gene mutation-growth phenotype relationship was verified by complementation analysis. The largest fraction of loci associated with temperature sensitivity were involved in the biosynthesis of the cell envelope, in particular the biosynthesis of extracellular polysaccharide. The largest fraction of loci associated with pressure sensitivity were involved in chromosomal structure and function. Genes for ribosome assembly and function were found to be important for both low-temperature and high-pressure growth. Likewise, both adaptation to temperature and adaptation to pressure were affected by mutations in a number of sensory and regulatory loci, suggesting the importance of signal transduction mechanisms in adaptation to either physical parameter. These analyses were the first global analyses of genes conditionally required for low-temperature or high-pressure growth in a deep-sea microorganism.

* Corresponding author. Mailing address: Center for Marine Biotechnology and Biomedicine, Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA 92093-0202. Phone: (858) 534-5233. Fax: (858) 534-7313. E-mail: dbartlett{at}ucsd.edu

{triangledown} Published ahead of print on 21 December 2007.

Journal of Bacteriology, March 2008, p. 1699-1709, Vol. 190, No. 5
0021-9193/08/$08.00+0     doi:10.1128/JB.01176-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • El-Hajj, Z. W., Tryfona, T., Allcock, D. J., Hasan, F., Lauro, F. M., Sawyer, L., Bartlett, D. H., Ferguson, G. P. (2009). Importance of Proteins Controlling Initiation of DNA Replication in the Growth of the High-Pressure-Loving Bacterium Photobacterium profundum SS9. J. Bacteriol. 191: 6383-6393 [Abstract] [Full Text]  
  • Eloe, E. A., Lauro, F. M., Vogel, R. F., Bartlett, D. H. (2008). The Deep-Sea Bacterium Photobacterium profundum SS9 Utilizes Separate Flagellar Systems for Swimming and Swarming under High-Pressure Conditions. Appl. Environ. Microbiol. 74: 6298-6305 [Abstract] [Full Text]  


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