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Journal of Bacteriology, March 2008, p. 2014-2022, Vol. 190, No. 6
0021-9193/08/$08.00+0     doi:10.1128/JB.01687-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Functional Consequences of Amino Acid Substitutions to GerVB, a Component of the Bacillus megaterium Spore Germinant Receptor{triangledown}

Graham Christie,* Milena Lazarevska, and Christopher R. Lowe

Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, United Kingdom

Received 19 October 2007/ Accepted 3 January 2008

The extreme metabolic dormancy and resistance properties of spores formed by members of the Bacillus and Clostridium genera are lost upon exposure to a variety of small-molecule germinants. Germinants are known to interact in an as yet undefined manner with cognate receptor complexes that reside in the inner membrane that surrounds the spore protoplast. The receptor itself is a complex of at least three proteins, and in this study we identify amino acid residues, predicted to lie in loop regions of GerVB on the exterior aspect of the membrane, that influence the Bacillus megaterium spore germination response. Three consecutive residues adjacent to putative transmembrane domain 10 (TM10) were demonstrated to mediate to various degrees the proline germinative response while also influencing germination in response to leucine, glucose, and inorganic salts, suggesting that this region may be part of a ligand binding pocket. Alternatively, substitutions in this region may affect the conformation of associated functionally important TM regions. Leucine- and KBr-mediated germination was also influenced by substitutions in other outer loop regions. These observations, when considered with accompanying kinetic analyses that demonstrate cooperativity between germinants, suggest that binding sites for the respective germinants are in close spatial proximity in the receptor but do not overlap. Additionally, proline recognition was conferred to a chimeric receptor when TM regions associated with the putative binding loop were present, indicating that residues in TM9 and/or TM10 of GerVB are also of functional importance in the proline-induced germinative response.

* Corresponding author. Mailing address: Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, United Kingdom. Phone: 44 1223 764959. Fax: 44 1223 334162. E-mail: gc301{at}cam.ac.uk

{triangledown} Published ahead of print on 18 January 2008.

Journal of Bacteriology, March 2008, p. 2014-2022, Vol. 190, No. 6
0021-9193/08/$08.00+0     doi:10.1128/JB.01687-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Christie, G., Lowe, C. R. (2008). Amino Acid Substitutions in Transmembrane Domains 9 and 10 of GerVB That Affect the Germination Properties of Bacillus megaterium Spores. J. Bacteriol. 190: 8009-8017 [Abstract] [Full Text]  


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