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Journal of Bacteriology, March 2008, p. 2217-2220, Vol. 190, No. 6
0021-9193/08/$08.00+0     doi:10.1128/JB.01778-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Identification of the Archaeal alg7 Gene Homolog (Encoding N-Acetylglucosamine-1-Phosphate Transferase) of the N-Linked Glycosylation System by Cross-Domain Complementation in Saccharomyces cerevisiae{triangledown}

Hosam Shams-Eldin,1,{dagger} Bonnie Chaban,2,{dagger} Sebastian Niehus,1,{ddagger} Ralph T. Schwarz,1,3 and Ken F. Jarrell2*

Institute for Virology, AG Parasitologie BMFZ, Philipps-University Marburg, Hans-Meerwein-Str. 2, 35043 Marburg, Germany,1 Department of Microbiology and Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada,2 Unité Glycobiologie Structurale et Fonctionnelle UMR CNRS/USTL n 8576 - IFR 147, Université Sciences et Technologies de Lille, 59655 Villeneuve D'Ascq cedex, France3

Received 9 November 2007/ Accepted 23 December 2007

The Mv1751 gene product is thought to catalyze the first step in the N-glycosylation pathway in Methanococcus voltae. Here, we show that a conditional lethal mutation in the alg7 gene (N-acetylglucosamine-1-phosphate transferase) in Saccharomyces cerevisiae was successfully complemented with Mv1751, highlighting a rare case of cross-domain complementation.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada. Phone: (613) 533-2456. Fax: (613) 533-6796. E-mail: jarrellk{at}queensu.ca

{triangledown} Published ahead of print on 4 January 2008.

{dagger} Both authors contributed equally to this work.

{ddagger} Present address: UPR 9022 du CNRS, Institut de Biologie Moléculaire et Cellulaire, 15 rue René Descartes, 67084 Strasbourg Cedex, France.

Journal of Bacteriology, March 2008, p. 2217-2220, Vol. 190, No. 6
0021-9193/08/$08.00+0     doi:10.1128/JB.01778-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Yurist-Doutsch, S., Eichler, J. (2009). Manual Annotation, Transcriptional Analysis, and Protein Expression Studies Reveal Novel Genes in the agl Cluster Responsible for N Glycosylation in the Halophilic Archaeon Haloferax volcanii. J. Bacteriol. 191: 3068-3075 [Abstract] [Full Text]  
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