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Journal of Bacteriology, March 2008, p. 2217-2220, Vol. 190, No. 6
0021-9193/08/$08.00+0     doi:10.1128/JB.01778-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Identification of the Archaeal alg7 Gene Homolog (Encoding N-Acetylglucosamine-1-Phosphate Transferase) of the N-Linked Glycosylation System by Cross-Domain Complementation in Saccharomyces cerevisiae

Hosam Shams-Eldin,^1, Bonnie Chaban,^2, Sebastian Niehus,^1, Ralph T. Schwarz,^1,3 and Ken F. Jarrell^2*

Institute for Virology, AG Parasitologie BMFZ, Philipps-University Marburg, Hans-Meerwein-Str. 2, 35043 Marburg, Germany,¹ Department of Microbiology and Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada,² Unité Glycobiologie Structurale et Fonctionnelle UMR CNRS/USTL n 8576 - IFR 147, Université Sciences et Technologies de Lille, 59655 Villeneuve D'Ascq cedex, France³

Received 9 November 2007/ Accepted 23 December 2007

The Mv1751 gene product is thought to catalyze the first step in the N-glycosylation pathway in Methanococcus voltae. Here, we show that a conditional lethal mutation in the alg7 gene (N-acetylglucosamine-1-phosphate transferase) in Saccharomyces cerevisiae was successfully complemented with Mv1751, highlighting a rare case of cross-domain complementation.

Published ahead of print on 4 January 2008.

Both authors contributed equally to this work.

Present address: UPR 9022 du CNRS, Institut de Biologie Moléculaire et Cellulaire, 15 rue René Descartes, 67084 Strasbourg Cedex, France.

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