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Journal of Bacteriology, March 2008, p. 2231-2238, Vol. 190, No. 6
0021-9193/08/$08.00+0     doi:10.1128/JB.01524-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

DNA Microarray Analysis of Central Carbohydrate Metabolism: Glycolytic/Gluconeogenic Carbon Switch in the Hyperthermophilic Crenarchaeum Thermoproteus tenax{triangledown} ,{dagger}

Melanie Zaparty,1* Alexander Zaigler,2,{ddagger} Claudia Stamme,2 Jörg Soppa,2 Reinhard Hensel,1 and Bettina Siebers1

Department of Biology and Geography, Institute of Biology, Microbiology I, University of Duisburg-Essen, Universitätsstr. 5, 45117 Essen, Germany,1 Biocenter Niederursel, Institute of Microbiology, Goethe-University, Max-von-Laue-Str. 9, 60438 Frankfurt, Germany2

Received 21 September 2007/ Accepted 23 December 2007

In order to unravel the role of regulation on transcript level in central carbohydrate metabolism (CCM) of Thermoproteus tenax, a focused DNA microarray was constructed by using 85 open reading frames involved in CCM. A transcriptional analysis comparing heterotrophic growth on glucose versus autotrophic growth on CO2-H2 was performed.

* Corresponding author. Mailing address: Department of Biology and Geography, Institute of Biology, Microbiology I, University of Duisburg-Essen, Universitätsstr. 5, 45117 Essen, Germany. Phone: 49 (0)201 1834366. Fax: 49 (0)201 1833990. E-mail: melanie.zaparty{at}uni-due.de

{triangledown} Published ahead of print on 4 January 2008.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

{ddagger} Present address: GANYMED Pharmaceuticals, Freiligrathstr. 12, 55131 Mainz, Germany.

Journal of Bacteriology, March 2008, p. 2231-2238, Vol. 190, No. 6
0021-9193/08/$08.00+0     doi:10.1128/JB.01524-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ramos-Vera, W. H., Berg, I. A., Fuchs, G. (2009). Autotrophic Carbon Dioxide Assimilation in Thermoproteales Revisited. J. Bacteriol. 191: 4286-4297 [Abstract] [Full Text]  


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