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"Candidatus Cloacamonas Acidaminovorans": Genome Sequence Reconstruction Provides a First Glimpse of a New Bacterial Division ^,

Eric Pelletier,^1,2,¶ Annett Kreimeyer,^1,2,¶ Stéphanie Bocs,^1,2, Zoé Rouy,^1,2 Gábor Gyapay,^1,2 Rakia Chouari,^1,2, Delphine Rivière,^1,2,3,4 Akila Ganesan,^1,2 Patrick Daegelen,^1,2 Abdelghani Sghir,^1,2,3 Georges N. Cohen,⁵ Claudine Médigue,^1,2 Jean Weissenbach,^1,2,3 and Denis Le Paslier^1,2*

CEA/Genoscope,¹ CNRS-UMR8030,² Université d'Evry Val d'Essonne, 91057 Evry, France,³ Suez-Environnement CIRSEE, 78230 Le Pecq, France,⁴ Institut Pasteur, 75015 Paris, France⁵

Received 2 August 2007/ Accepted 14 January 2008

Many microorganisms live in anaerobic environments. Most of these microorganisms have not yet been cultivated. Here, we present, from a metagenomic analysis of an anaerobic digester of a municipal wastewater treatment plant, a reconstruction of the complete genome of a bacterium belonging to the WWE1 candidate division. In silico proteome analysis indicated that this bacterium might derive most of its carbon and energy from the fermentation of amino acids, and hence, it was provisionally classified as "Candidatus Cloacamonas acidaminovorans." "Candidatus Cloacamonas acidaminovorans" is probably a syntrophic bacterium that is present in many anaerobic digesters. This report highlights how environmental sequence data might provide genomic and functional information about a new bacterial clade whose members are involved in anaerobic digestion.

Published ahead of print on 1 February 2008.

Supplemental material for this article may be found at http://jb.asm.org/.

^¶ E.P. and A.K. contributed equally to this work.

Present address: UMR DAP-CIRAD, 34398 Montpellier Cedex 5, France.

Present address: Faculté des Sciences de Bizerte, 7021 Zarzouna, Bizerte, Tunisia.