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Journal of Bacteriology, April 2008, p. 2588-2596, Vol. 190, No. 7
0021-9193/08/$08.00+0     doi:10.1128/JB.01538-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Three-Dimensional Imaging of the Highly Bent Architecture of Bdellovibrio bacteriovorus by Using Cryo-Electron Tomography{triangledown} ,{dagger}

Mario J. Borgnia, Sriram Subramaniam, and Jacqueline L. S. Milne*

Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892

Received 24 September 2007/ Accepted 7 January 2008

Bdellovibrio bacteriovorus cells are small deltaproteobacterial cells that feed on other gram-negative bacteria, including human pathogens. Using cryo-electron tomography, we demonstrated that B. bacteriovorus cells are capable of substantial flexibility and local deformation of the outer and inner membranes without loss of cell integrity. These shape changes can occur in less than 2 min, and analysis of the internal architecture of highly bent cells showed that the overall distribution of molecular machines and the nucleoid is similar to that in moderately bent cells. B. bacteriovorus cells appear to contain an extensive internal network of short and long filamentous structures. We propose that rearrangements of these structures, in combination with the unique properties of the cell envelope, may underlie the remarkable ability of B. bacteriovorus cells to find and enter bacterial prey.

* Corresponding author. Mailing address: Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Building 50, Room 4306, 50 South Drive, Bethesda, MD 20892. Phone: (301) 594-2063. Fax: (301) 480-3834. E-mail: jmilne{at}nih.gov

{triangledown} Published ahead of print on 18 January 2008.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, April 2008, p. 2588-2596, Vol. 190, No. 7
0021-9193/08/$08.00+0     doi:10.1128/JB.01538-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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