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Journal of Bacteriology, April 2008, p. 2611-2614, Vol. 190, No. 7
0021-9193/08/$08.00+0     doi:10.1128/JB.01896-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Dissection of Ammonium Uptake Systems in Corynebacterium glutamicum: Mechanism of Action and Energetics of AmtA and AmtB{triangledown}

Britta Walter,1 Melanie Küspert,1 Daniel Ansorge,2 Reinhard Krämer,2* and Andreas Burkovski1*

Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, Germany,1 Institut für Biochemie der Universität zu Köln, Zülpicher-Str. 47, 50674 Köln, Germany2

Received 4 December 2007/ Accepted 18 January 2008

Corynebacterium glutamicum has two different Amt-type proteins. While AmtB has a low substrate affinity and is not saturable up to 3 mM methylammonium, AmtA has a high substrate affinity and mediates saturable, membrane potential-dependent transport, resulting in a high steady-state accumulation of methylammonium, even in the absence of metabolic trapping.

* Corresponding author. Mailing address for Reinhard Krämer: Institut für Biochemie, Universität zu Köln, Zülpicher-Str. 47, 50674 Köln, Germany. Phone: 49 221 470 6461. Fax: 49 221 470 5091. E-mail: r.kraemer{at}uni-koeln.de. Mailing address for Andreas Burkovski: Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstr. 5, 91058 Erlangen, Germany. Phone: 49 9131 85 28086. Fax: 49 9131 85 28082. E-mail: aburkov{at}biologie.uni-erlangen.de

{triangledown} Published ahead of print on 1 February 2008.

Journal of Bacteriology, April 2008, p. 2611-2614, Vol. 190, No. 7
0021-9193/08/$08.00+0     doi:10.1128/JB.01896-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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