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Journal of Bacteriology, April 2008, p. 2629-2632, Vol. 190, No. 7
0021-9193/08/$08.00+0     doi:10.1128/JB.01722-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Novel Monofunctional Histidinol-Phosphate Phosphatase of the DDDD Superfamily of Phosphohydrolases{triangledown}

Hyun Sook Lee,{dagger} Yona Cho,{dagger} Jung-Hyun Lee, and Sung Gyun Kang*

Korea Ocean Research & Development Institute, Ansan P.O. Box 29, Seoul 425-600, Republic of Korea

Received 28 October 2007/ Accepted 11 January 2008

The TON_0887 gene was identified as the missing histidinol-phosphate phosphatase (HolPase) in the hyperthermophilic archaeon "Thermococcus onnurineus" NA1. The protein contained conserved motifs of the DDDD superfamily of phosphohydrolase, and the recombinantly expressed protein exhibited strong HolPase activity. In this study, we functionally assessed for the first time the monofunctional DDDD-type HolPase, which is organized in the gene cluster.

* Corresponding author. Mailing address: Korea Ocean Research & Development Institute, Ansan P.O. Box 29, Seoul 425-600, Republic of Korea. Phone: 82-31-400-6241. Fax: 82-31-406-2495. E-mail: sgkang{at}kordi.re.kr

{triangledown} Published ahead of print on 25 January 2008.

{dagger} These authors contributed equally to this work.

Journal of Bacteriology, April 2008, p. 2629-2632, Vol. 190, No. 7
0021-9193/08/$08.00+0     doi:10.1128/JB.01722-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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