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Journal of Bacteriology, April 2008, p. 2851-2857, Vol. 190, No. 8
0021-9193/08/$08.00+0     doi:10.1128/JB.01904-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

SprB Is a Cell Surface Component of the Flavobacterium johnsoniae Gliding Motility Machinery{triangledown} ,{dagger}

Shawn S. Nelson, Sreelekha Bollampalli, and Mark J. McBride*

Department of Biological Sciences, University of Wisconsin-Milwaukee, P.O. Box 413, Milwaukee, Wisconsin 53201

Received 5 December 2007/ Accepted 6 February 2008

Cells of the gliding bacterium Flavobacterium johnsoniae move rapidly over surfaces by an unknown mechanism. Transposon insertions in sprB resulted in cells that were defective in gliding. SprB is a highly repetitive 669-kDa cell surface protein, and antibodies against SprB inhibited the motility of wild-type cells. Polystyrene microspheres coated with antibodies against SprB attached to and were rapidly propelled along the cell surface, suggesting that SprB is one of the outermost components of the motility machinery. The movement of SprB along the cell surface supports a model of gliding motility in which motors anchored to the cell wall rapidly propel cell surface adhesins.

* Corresponding author. Mailing address: Department of Biological Sciences, 181 Lapham Hall, University of Wisconsin-Milwaukee, 3209 N. Maryland Ave., Milwaukee, WI 53211. Phone: (414) 229-5844. Fax: (414) 229-3926. E-mail: mcbride{at}uwm.edu

{triangledown} Published ahead of print on 15 February 2008.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, April 2008, p. 2851-2857, Vol. 190, No. 8
0021-9193/08/$08.00+0     doi:10.1128/JB.01904-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • McBride, M. J., Xie, G., Martens, E. C., Lapidus, A., Henrissat, B., Rhodes, R. G., Goltsman, E., Wang, W., Xu, J., Hunnicutt, D. W., Staroscik, A. M., Hoover, T. R., Cheng, Y.-Q., Stein, J. L. (2009). Novel Features of the Polysaccharide-Digesting Gliding Bacterium Flavobacterium johnsoniae as Revealed by Genome Sequence Analysis. Appl. Environ. Microbiol. 75: 6864-6875 [Abstract] [Full Text]  


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