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Journal of Bacteriology, January 2009, p. 196-202, Vol. 191, No. 1
0021-9193/09/$08.00+0     doi:10.1128/JB.01264-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Relaxed Specificity of the Bacillus subtilis TatAdCd Translocase in Tat-Dependent Protein Secretion{triangledown}

Robyn T. Eijlander, Jan D. H. Jongbloed,§ and Oscar P. Kuipers*

Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Haren, The Netherlands

Received 9 September 2008/ Accepted 23 October 2008

Protein translocation via the twin arginine translocation (TAT) pathway is characterized by the translocation of prefolded proteins across the hydrophobic lipid bilayer of the membrane. In Bacillus subtilis, two different Tat translocases are involved in this process, and both display different substrate specificities: PhoD is secreted via TatAdCd, whereas YwbN is secreted via TatAyCy. It was previously assumed that both TatAy and TatCy are essential for the translocation of the YwbN precursor. Through complementation studies, we now show that TatAy can be functionally replaced by TatAd when the latter is offered to the cells in excess amounts. Moreover, under conditions of overproduction, TatAdCd, in contrast to TatAyCy, shows an increased tolerance toward the acceptance of various Tat-dependent proteins.

* Corresponding author. Mailing address: University of Groningen, Department of Genetics, Kerklaan 30, Haren 9751 NN, The Netherlands. Phone: 31 503632093. Fax: 31 503632348. E-mail: o.p.kuipers{at}rug.nl

{triangledown} Published ahead of print on 31 October 2008.

§ Present address: Department of Genetics, University Medical Center of Groningen, Groningen, The Netherlands.

Journal of Bacteriology, January 2009, p. 196-202, Vol. 191, No. 1
0021-9193/09/$08.00+0     doi:10.1128/JB.01264-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ridder, A. N. J. A., de Jong, E. J., Jongbloed, J. D. H., Kuipers, O. P. (2009). Subcellular Localization of TatAd of Bacillus subtilis Depends on the Presence of TatCd or TatCy. J. Bacteriol. 191: 4410-4418 [Abstract] [Full Text]  
  • Eijlander, R. T., Kolbusz, M. A., Berendsen, E. M., Kuipers, O. P. (2009). Effects of altered TatC proteins on protein secretion efficiency via the twin-arginine translocation pathway of Bacillus subtilis. Microbiology 155: 1776-1785 [Abstract] [Full Text]  


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