This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hirooka, K.
Right arrow Articles by Fujita, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hirooka, K.
Right arrow Articles by Fujita, Y.

 Previous Article  |  Next Article 

Journal of Bacteriology, June 2009, p. 3685-3697, Vol. 191, No. 11
0021-9193/09/$08.00+0     doi:10.1128/JB.00202-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Regulation of the Bacillus subtilis Divergent yetL and yetM Genes by a Transcriptional Repressor, YetL, in Response to Flavonoids{triangledown}

Kazutake Hirooka, Yusuke Danjo, Yuki Hanano, Satoshi Kunikane, Hiroshi Matsuoka, Shigeo Tojo, and Yasutaro Fujita*

Department of Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama University, 985 Sanzo, Higashimura, Fukuyama 729-0292, Japan

Received 16 February 2009/ Accepted 18 March 2009

DNA microarray analysis revealed that transcription of the Bacillus subtilis yetM gene encoding a putative flavin adenine dinucleotide-dependent monooxygenase was triggered by certain flavonoids during culture and was derepressed by disruption of the yetL gene in the opposite orientation situated immediately upstream of yetM, which encodes a putative MarR family transcriptional regulator. In vitro analyses, including DNase I footprinting and gel retardation analysis, indicated that YetL binds specifically to corresponding single sites in the divergent yetL and yetM promoter regions, with higher affinity to the yetM region; the former region overlaps the Shine-Dalgarno sequence of yetL, and the latter region contains a perfect 18-bp palindromic sequence (TAGTTAGGCGCCTAACTA). In vitro gel retardation and in vivo lacZ fusion analyses indicated that some flavonoids (kaempferol, apigenin, and luteolin) effectively inhibit YetL binding to the yetM cis sequence, but quercetin, galangin, and chrysin do not inhibit this binding, implying that the 4-hydroxyl group on the B-ring of the flavone structure is indispensable for this inhibition and that the coexistence of the 3-hydroxyl groups on the B- and C-rings does not allow antagonism of YetL.

* Corresponding author. Mailing address: Department of Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama University, 985 Sanzo, Higashimura, Fukuyama 729-0292, Japan. Phone: 81-84-936-2111. Fax: 81-84-936-2023. E-mail: yfujita{at}bt.fubt.fukuyama-u.ac.jp

{triangledown} Published ahead of print on 27 March 2009.

Journal of Bacteriology, June 2009, p. 3685-3697, Vol. 191, No. 11
0021-9193/09/$08.00+0     doi:10.1128/JB.00202-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»