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Journal of Bacteriology, June 2009, p. 3747-3751, Vol. 191, No. 11
0021-9193/09/$08.00+0     doi:10.1128/JB.00069-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Promoter Characterization in the AT-Rich Genome of the Obligate Endosymbiont "Candidatus Blochmannia floridanus"{triangledown}

Sascha Stoll,1 Heike Feldhaar,2 and Roy Gross1*

Lehrstuhl für Mikrobiologie, Biozentrum, Universität Würzburg, Am Hubland, D-97074 Würzburg, Germany,1 Verhaltensbiologie, Universität Osnabrück, Barbarastraße 11, D-49076 Osnabrück, Germany2

Received 20 January 2009/ Accepted 16 March 2009

The characterization of transcriptional start sites of 14 genes encoded by the extremely AT-rich genome of "Candidatus Blochmannia floridanus" revealed a high degree of conservation with the RpoD promoter consensus sequence of the free-living relative Escherichia coli. Moreover, in agreement with the presence of the alternative heat shock sigma factor RpoH in "Ca. Blochmannia," typical RpoH-dependent promoters were identified. However, no heat shock response resembling that of E. coli could be detected in "Ca. Blochmannia."

* Corresponding author. Mailing address: Theodor-Boveri-Institut für Biowissenschaften, Lehrstuhl für Mikrobiologie, Universität Würzburg, Am Hubland, D-97074 Würzburg, Germany. Phone: (931) 318 4403. Fax: (931) 888 4402. E-mail: roy{at}biozentrum.uni-wuerzburg.de

{triangledown} Published ahead of print on 27 March 2009.

Journal of Bacteriology, June 2009, p. 3747-3751, Vol. 191, No. 11
0021-9193/09/$08.00+0     doi:10.1128/JB.00069-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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