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Journal of Bacteriology, June 2009, p. 4025-4029, Vol. 191, No. 12
0021-9193/09/$08.00+0     doi:10.1128/JB.00118-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Genomic Sequencing Reveals Regulatory Mutations and Recombinational Events in the Widely Used MC4100 Lineage of Escherichia coli K-12{triangledown} ,{dagger}

Thomas Ferenci,3 Zhemin Zhou,1 Thu Betteridge,3 Yan Ren,1 Yu Liu,1 Lu Feng,1,2 Peter R. Reeves,3 and Lei Wang1,2,4*

TEDA School of Biological Sciences and Biotechnology, Nankai University,1 Tianjin Research Center for Functional Genomics and Biochip, 23 Hongda Street, Tianjin Economic-Technological Development Area (TEDA), Tianjin 300457, China,2 School of Molecular and Microbial Biosciences, University of Sydney, Sydney, New South Wales, Australia,3 Tianjin Key Laboratory of Microbial Functional Genomics, Nankai University, 23 Hongda Street, Tianjin Economic-Technological Development Area (TEDA), Tianjin 300457, China4

Received 28 January 2009/ Accepted 6 April 2009

The genome of an Escherichia coli MC4100 strain with a {lambda} placMu50 fusion revealed numerous regulatory differences from MG1655, including one that arose during laboratory storage. The 194 mutational differences between MC4100(MuLac) and other K-12 sequences were mostly allocated to specific lineages, indicating the considerable mutational divergence between K-12 strains.

* Corresponding author. Mailing address: TEDA School of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, TEDA, Tianjin 300457, People's Republic of China. Phone: 86-22-66229588. Fax: 86-22-66229596. E-mail: wanglei{at}nankai.edu.cn

{triangledown} Published ahead of print on 17 April 2009.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, June 2009, p. 4025-4029, Vol. 191, No. 12
0021-9193/09/$08.00+0     doi:10.1128/JB.00118-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



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