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Journal of Bacteriology, July 2009, p. 4152-4157, Vol. 191, No. 13
0021-9193/09/$08.00+0     doi:10.1128/JB.00227-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

The Quorum Sensing-Dependent Gene katG of Burkholderia glumae Is Important for Protection from Visible Light{triangledown}

Heejin Chun,1 Okhee Choi,1 Eunhye Goo,1 Nayeon Kim,1 Hongsup Kim,1 Yongsung Kang,1 Jinwoo Kim,1 Jae Sun Moon,2 and Ingyu Hwang1*

Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University, Seoul 151-921, Republic of Korea,1 Plant Genome Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-633, Republic of Korea2

Received 19 February 2009/ Accepted 21 April 2009

Quorum sensing (QS) plays important roles in the pathogenicity of Burkholderia glumae, the causative agent of bacterial rice grain rot. We determined how QS is involved in catalase expression in B. glumae. The QS-defective mutant of B. glumae exhibited less catalase activity than wild-type B. glumae. A β-glucuronidase assay of a katG::Tn3-gusA78 reporter fusion protein revealed that katG expression is under the control of QS. Furthermore, katG expression was upregulated by QsmR, a transcriptional activator for flagellar-gene expression that is regulated by QS. A gel mobility shift assay confirmed that QsmR directly activates katG expression. The katG mutant produced toxoflavin but exhibited less severe disease than BGR1 on rice panicles. Under visible light conditions and a photon flux density of 61.6 µmol–1 m–2, the survival rate of the katG mutant was 105-fold lower than that of BGR1. This suggests that KatG is a major catalase that protects bacterial cells from visible light, which probably results in less severe disease caused by the katG mutant.

* Corresponding author. Mailing address: Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea. Phone: 82-2-880-4676. Fax: 82-2-873-2317. E-mail: ingyu{at}snu.ac.kr

{triangledown} Published ahead of print on 24 April 2009.

Journal of Bacteriology, July 2009, p. 4152-4157, Vol. 191, No. 13
0021-9193/09/$08.00+0     doi:10.1128/JB.00227-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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