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Journal of Bacteriology, July 2009, p. 4383-4391, Vol. 191, No. 13
0021-9193/09/$08.00+0     doi:10.1128/JB.00183-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Characterization of an Alcohol Dehydrogenase from the Cyanobacterium Synechocystis sp. Strain PCC 6803 That Responds to Environmental Stress Conditions via the Hik34-Rre1 Two-Component System{triangledown} ,{dagger}

Rebeca Vidal, Luis López-Maury,{ddagger} Miguel G. Guerrero, and Francisco J. Florencio*

Instituto de Bioquímica Vegetal y Fotosíntesis, CSIC—Universidad de Sevilla, Av. Américo Vespucio 49, E-41092 Sevilla, Spain

Received 11 February 2009/ Accepted 22 April 2009

The slr1192 (adhA) gene from Synechocystis sp. strain PCC 6803 encodes a member of the medium-chain alcohol dehydrogenase/reductase family. The gene product AdhA exhibits NADP-dependent alcohol dehydrogenase activity, acting on a broad variety of aromatic and aliphatic primary alcohols and aldehydes but not on secondary alcohols or ketones. It exhibits superior catalytic efficiency for aldehyde reduction compared to that for alcohol oxidation. The enzyme is a cytosolic protein present in photoautotrophically grown Synechocystis cells. The expression of AdhA is enhanced upon the exposure of cells to different environmental stresses, although it is not essential for survival even under such stress conditions. The induction of the expression of the adhA gene is dependent on the Hik34-Rre1 two-component system, as it is severely impaired in mutant strains lacking either the histidine kinase Hik34 or the response regulator Rre1. In vitro DNA-protein interaction analysis reveals that the response regulator Rre1 binds specifically to the promoter region of the adhA gene.

* Corresponding author. Mailing address: Instituto de Bioquímica Vegetal y Fotosíntesis, Universidad de Sevilla—CSIC, Av. Americo Vespucio 49, E-41092 Seville, Spain. Phone: 34 954489509. Fax: 34 954460065. E-mail: floren{at}us.es

{triangledown} Published ahead of print on 1 May 2009.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

{ddagger} Present address: Fission Yeast Functional Genomics, Cancer Research UK, Wellcome Trust Sanger Institute, Morgan Building, Hinxton, Cambridge CB10 1HH, United Kingdom.

Journal of Bacteriology, July 2009, p. 4383-4391, Vol. 191, No. 13
0021-9193/09/$08.00+0     doi:10.1128/JB.00183-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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