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Journal of Bacteriology, July 2009, p. 4487-4491, Vol. 191, No. 14
0021-9193/09/$08.00+0     doi:10.1128/JB.00056-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Dominant Negative Autoregulation Limits Steady-State Repression Levels in Gene Networks{triangledown} ,{dagger}

Szabolcs Semsey,1* Sandeep Krishna,2 János Erdossy,1 Péter Horváth,1 László Orosz,1 Kim Sneppen,2 and Sankar Adhya3

Department of Genetics, Eötvös Lóránd University, H-1117 Budapest, Hungary,1 Center for Models of Life, Niels Bohr Institute, Copenhagen, Denmark,2 Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-42643

Received 16 January 2009/ Accepted 3 May 2009

Many transcription factors repress transcription of their own genes. Negative autoregulation has been shown to reduce cell-cell variation in regulatory protein levels and speed up the response time in gene networks. In this work we examined transcription regulation of the galS gene and the function of its product, the GalS protein. We observed a unique operator preference of the GalS protein characterized by dominant negative autoregulation. We show that this pattern of regulation limits the repression level of the target genes in steady states. We suggest that transcription factors with dominant negative autoregulation are designed for regulating gene expression during environmental transitions.

* Corresponding author. Mailing address: Department of Genetics, Eötvös Lóránd University, Pázmány P. s. 1/C, H-1117 Budapest, Hungary. Phone: 36 (1) 3722500. Fax: 36 (1) 3722641. E-mail: semseys{at}yahoo.com

{triangledown} Published ahead of print on 12 May 2009.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, July 2009, p. 4487-4491, Vol. 191, No. 14
0021-9193/09/$08.00+0     doi:10.1128/JB.00056-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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