This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Google Scholar
Right arrow Articles by Shimada, T.
Right arrow Articles by Ishihama, A.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shimada, T.
Right arrow Articles by Ishihama, A.

 Previous Article  |  Next Article 

Journal of Bacteriology, July 2009, p. 4562-4571, Vol. 191, No. 14
0021-9193/09/$08.00+0     doi:10.1128/JB.00108-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Involvement of the Leucine Response Transcription Factor LeuO in Regulation of the Genes for Sulfa Drug Efflux{triangledown} ,{dagger}

Tomohiro Shimada, Kaneyoshi Yamamoto, and Akira Ishihama*

Department of Frontier Bioscience and Micro-Nano Technology Research Center, Hosei University, Koganei, Tokyo 184-8584, Japan

Received 26 January 2009/ Accepted 1 May 2009

LeuO, a LysR family transcription factor, exists in a wide variety of bacteria of the family Enterobacteriaceae and is involved in the regulation of as yet unidentified genes affecting the stress response and pathogenesis expression. Using genomic screening by systematic evolution of ligands by exponential enrichment (SELEX) in vitro, a total of 106 DNA sequences were isolated from 12 different regions of the Escherichia coli genome. All of the SELEX fragments formed complexes in vitro with purified LeuO. After Northern blot analysis of the putative target genes located downstream of the respective LeuO-binding sequence, a total of nine genes were found to be activated by LeuO, while three genes were repressed by LeuO. The LeuO target gene collection included several multidrug resistance genes. A phenotype microarray assay was conducted to identify the gene(s) responsible for drug resistance and the drug species that are under the control of the LeuO target gene(s). The results described herein indicate that the yjcRQP operon, one of the LeuO targets, is involved in sensitivity control against sulfa drugs. We propose to rename the yjcRQP genes the sdsRQP genes (sulfa drug sensitivity determinant).

* Corresponding author. Mailing address: Department of Frontier Bioscience, Hosei University, Koganei, Tokyo 184-8584, Japan. Phone and fax: 81-42-387-6231. E-mail: aishiham{at}hosei.ac.jp

{triangledown} Published ahead of print on 1 May 2009.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, July 2009, p. 4562-4571, Vol. 191, No. 14
0021-9193/09/$08.00+0     doi:10.1128/JB.00108-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ishida, Y., Kori, A., Ishihama, A. (2009). Participation of Regulator AscG of the {beta}-Glucoside Utilization Operon in Regulation of the Propionate Catabolism Operon. J. Bacteriol. 191: 6136-6144 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»