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Journal of Bacteriology, August 2009, p. 5010-5012, Vol. 191, No. 15
0021-9193/09/$08.00+0     doi:10.1128/JB.00306-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Oleate Hydratase Catalyzes the Hydration of a Nonactivated Carbon-Carbon Bond{triangledown} ,{dagger}

Loes E. Bevers, Martijn W. H. Pinkse, Peter D. E. M. Verhaert, and Wilfred R. Hagen*

Department of Biotechnology, Delft University of Technology, Delft, The Netherlands

Received 6 March 2009/ Accepted 17 May 2009

The hydration of oleic acid into 10-hydroxystearic acid was originally described for a Pseudomonas cell extract almost half a century ago. In the intervening years, the enzyme has never been characterized in any detail. We report here the isolation and characterization of oleate hydratase (EC 4.2.1.53) from Elizabethkingia meningoseptica.

* Corresponding author. Mailing address: Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands. Phone: 31 15 2785051. Fax: 31 15 2782355. E-mail: w.r.hagen{at}tudelft.nl

{triangledown} Published ahead of print on 22 May 2009.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

Journal of Bacteriology, August 2009, p. 5010-5012, Vol. 191, No. 15
0021-9193/09/$08.00+0     doi:10.1128/JB.00306-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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