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Journal of Bacteriology, March 2009, p. 1986-1991, Vol. 191, No. 6
0021-9193/09/$08.00+0     doi:10.1128/JB.01606-07
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

ClpX Inhibits FtsZ Assembly in a Manner That Does Not Require Its ATP Hydrolysis-Dependent Chaperone Activity{triangledown} ,{dagger}

Daniel P. Haeusser,{ddagger},§ Amy H. Lee,{ddagger},¶ Richard B. Weart,# and Petra Anne Levin*

Department of Biology, Washington University, Campus Box 1137, One Brookings Drive, St. Louis, Missouri 63130

Received 3 October 2007/ Accepted 29 December 2008

ClpX is a well-characterized bacterial chaperone that plays a role in many processes, including protein turnover and the remodeling of macromolecular complexes. All of these activities require ATP hydrolysis-dependent, ClpX-mediated protein unfolding. Here we used site-directed mutagenesis in combination with genetics and biochemistry to establish that ClpX inhibits assembly of the conserved division protein FtsZ through a noncanonical mechanism independent of its role as an ATP-dependent chaperone.

* Corresponding author. Mailing address: Department of Biology, Washington University, Campus Box 1137, One Brookings Drive, St. Louis, MO 63130. Phone: (314) 935-7888. Fax: (314) 935-4432. E-mail: plevin{at}biology.wustl.edu

{triangledown} Published ahead of print on 9 January 2009.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

{ddagger} These authors contributed equally to this work.

§ Present address: Department of Biochemistry and Molecular Biology, Penn State University, University Park, PA 16801.

Present address: Department of Cell and Systems Biology, University of Toronto, Toronto, ON, Canada M5S 3B2.

# Present address: Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139.

Journal of Bacteriology, March 2009, p. 1986-1991, Vol. 191, No. 6
0021-9193/09/$08.00+0     doi:10.1128/JB.01606-07
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Camberg, J. L., Hoskins, J. R., Wickner, S. (2009). ClpXP protease degrades the cytoskeletal protein, FtsZ, and modulates FtsZ polymer dynamics. Proc. Natl. Acad. Sci. USA 106: 10614-10619 [Abstract] [Full Text]  


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