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Journal of Bacteriology, April 2009, p. 2371-2379, Vol. 191, No. 7
0021-9193/09/$08.00+0     doi:10.1128/JB.01525-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Complete Genome Sequence of the Anaerobic, Protein-Degrading Hyperthermophilic Crenarchaeon Desulfurococcus kamchatkensis{triangledown} ,{dagger}

Nikolai V. Ravin,1,{ddagger} Andrey V. Mardanov,1,{ddagger} Alexey V. Beletsky,1 Ilya V. Kublanov,2 Tatiana V. Kolganova,1 Alexander V. Lebedinsky,2 Nikolai A. Chernyh,2 Elizaveta A. Bonch-Osmolovskaya,2 and Konstantin G. Skryabin1*

Centre "Bioengineering," Russian Academy of Sciences, Moscow 117312, Russia,1 Winogradsky Institute of Microbiology, Russian Academy of Sciences, Moscow 117312, Russia2

Received 28 October 2008/ Accepted 23 December 2008

Desulfurococcus kamchatkensis is an anaerobic organotrophic hyperthermophilic crenarchaeon isolated from a terrestrial hot spring. Its genome consists of a single circular chromosome of 1,365,223 bp with no extrachromosomal elements. A total of 1,474 protein-encoding genes were annotated, among which 205 are exclusive for D. kamchatkensis. The search for a replication origin site revealed a single region coinciding with a global extreme of the nucleotide composition disparity curve and containing a set of crenarchaeon-type origin recognition boxes. Unlike in most archaea, two genes encoding homologs of the eukaryotic initiator proteins Orc1 and Cdc6 are located distantly from this site. A number of mobile elements are present in the genome, including seven transposons representing IS607 and IS200/IS605 families and multiple copies of miniature inverted repeat transposable elements. Two large clusters of regularly interspaced repeats are present; none of the spacer sequences matches known archaeal extrachromosomal elements, except one spacer matches the sequence of a resident gene of D. kamchatkensis. Many of the predicted metabolic enzymes are associated with the fermentation of peptides and sugars, including more than 30 peptidases with diverse specificities, a number of polysaccharide degradation enzymes, and many transporters. Consistently, the genome encodes both enzymes of the modified Embden-Meyerhof pathway of glucose oxidation and a set of enzymes needed for gluconeogenesis. The genome structure and content reflect the organism's nutritionally diverse, competitive natural environment, which is periodically invaded by viruses and other mobile elements.

* Corresponding author. Mailing address: Centre "Bioengineering," Russian Academy of Sciences, Prosp. 60-Let Oktyabrya, Bld. 7-1, Moscow 117312, Russia. Phone: (7) 499 7833264. Fax: (7) 499 1357319. E-mail: nravin{at}biengi.ac.ru

{triangledown} Published ahead of print on 29 December 2008.

{dagger} Supplemental material for this article may be found at http://jb.asm.org/.

{ddagger} N.V.R. and A.V.M. contributed equally to this work.

Journal of Bacteriology, April 2009, p. 2371-2379, Vol. 191, No. 7
0021-9193/09/$08.00+0     doi:10.1128/JB.01525-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mardanov, A. V., Ravin, N. V., Svetlitchnyi, V. A., Beletsky, A. V., Miroshnichenko, M. L., Bonch-Osmolovskaya, E. A., Skryabin, K. G. (2009). Metabolic Versatility and Indigenous Origin of the Archaeon Thermococcus sibiricus, Isolated from a Siberian Oil Reservoir, as Revealed by Genome Analysis. Appl. Environ. Microbiol. 75: 4580-4588 [Abstract] [Full Text]  


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